水稻DNA限制性片段长度多态性的初步研究

 应用19个不同的探针／限制性内切酶组合，测定了8个不同基因型水稻间的DNA限制性片段长度多态性(RFLP)。结果表明：RFLP在水稻中普遍存在，同一亚种内的RFLP较少，而在不同亚种间则较大；栽培稻与野生稻间的RFLP较多，而以粳稻和野生稻之间RFLP最多，达58％－68％。用IBM ／PC计算机分析了这些基因型之间的遗传距离，粳稻和籼稻可明显地分成二组，野生稻与籼稻的亲缘大于与粳稻的亲缘。具有广亲和能力的爪哇型品种Ketan Nangka偏向籼型。

Restriction Fragment Length Polymorphism in Rice

Eight genotypes of cultivated (Oryza sativa L.)and wild rice (Oryza glumaepatula)were surveyed for restriction fragment length polymorphisms (RFLPs) using 9 probes and 4 endonucteases, all together 19 probe / enzyme combinations. Results indicated that RFLPs existed generally in rice. The RFLPs were more obvious between subspecies than within the subspecies.The RFLPs between cultivated and wild rice were obvious. The RFLPs between japonica and wild rice were the greatest, which were 58-68%. Nei's average gene diversity was used as a measure of genetic variability for restriction fragment lengths within genotypes and a dendrogram was constructed from genetic distance estimates between genotypes. Genotypes of japonica and indica were grouped respectively. The indica was closer relative of wild rice than japonica. The javaniea variety Ketan Nangka, which showed wide compatibility, was more closer to indica.