| Abstract: | Cherry virus A(CVA) is a member of the genus Capillovirus, in the family Betaflexiviridae. The infection rate of CVA was high in sweet cherry in China. We determined the complete nucleotide sequences of two isolates of CVA from Tai'an, Shandong Province, China using high fidelity PCR enzymes and specific primer pairs for amplifying long fragments in RT-PCR and RACE. The full-length sequences from isolates Ch TA11 and Ch TA12 are both 7 382 nucleotide(nt) long, excluding the poly(A) tail, encode two open reading frames(ORFs) and have similar genome organization to the two isolates in GenBank. The complete nucleotide sequence of Ch TA11 is 98.2 and 81.2% nt identity to t he isolates from Germany and India in Gen Bank, respectively, and the Ch TA12 isolate is 98.2 and 81.0% similar. Analysis of the nucleotide and amino acid sequences showed that the domain of unknown function(DUF1717) is more variable compared with other domains. This is the first report of the complete nucleotide sequences of CVA isolates infecting sweet cherry in China. |
