花生(Arachis hypogaea L.) CaM基因克隆与序列分析

提取花生(Arachis hypogaea L.)幼嫩叶片基因组DNA,合成5'端和3'端引物,进行PCR并克隆测序,得到CaM基因组的2个异型基因PGCaM-1和PGCaM-3,登录Genebank并注册为AY517933、AY572856。序列分析表明,PGCaM-1序列全长1425 bp,在第25个氨基酸之后有一个长度为973 bp的内含子,PGCaM-3序列全长447 bp。两个异型基因的开放读码框均由447个核苷酸组成,共编码148个氨基酸,同属于EF手型CaM超家族成员,核苷酸和氨基酸序列的同源性分别为96%和98%,与已知花生CaM cDNA序列及氨基酸均有极高的同源性,序列之间的差异可能引起表达和功能上的差异。

Cloning of Two Genomics Genes and Sequence Analysis Encoding Calmodulin from Peanut (Arachis hypogaea L.)

Two heteromotphic CaM genes in genomics of peanut(Arachis hypogaea L.) leaflet were cloned by PCR reaction using primers synthesized accord to the CaM conservative sequences and registered in Genebank as AY517933 and AY572856 respectively.Result showed that the whole length of PGCaM-1 gene was 1425 base pairs and had a 973 base pairs intron behind the 25th amino acid while PGCaM-3 gene was 447 base pairs.Both of their open reading frame were composed of 447 base pairs encoding 148 amino acids and sequence identity between them were 96% and 98% respectively.In addition, they both belonged to the EF-Hand super-family of calmodulin and also shared high homology with known peanut calmodulin cDNA sequence and other higher plants,The title differences maybe result in different expressions and functions.