| 转人乳铁蛋白基因山羊乳腺上皮细胞的研究 |
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| 引用本文： | 郑月茂,刘凤军,何小宁,张涌. 转人乳铁蛋白基因山羊乳腺上皮细胞的研究[J]. 畜牧兽医学报, 2006, 37(12): 1282-1286 |
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| 作者姓名： | 郑月茂 刘凤军 何小宁 张涌 |
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| 作者单位： | 农业部家畜生殖内分泌与胚胎工程重点开放实验室,西北农林科技大学生物工程研究所,杨凌,712100 |
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| 基金项目： | 西北农林科技大学人才基金资助项目;国家科技部“863”高技术研究发展规划资助项目（2001AA213081） |
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| 摘 要： | 利用脂质体包裹含人乳铁蛋白基因的重组质粒，并导入到山羊乳腺上皮细胞，经G418及PCR筛选获得阳性细胞；转染细胞增殖后经激素诱导，培养液上清通过Western blot检测证明，转染细胞表达并分泌出人乳铁蛋白，其分子质量为58ku。
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| 关 键 词： | 人乳铁蛋白基因乳腺特异性表达载体 绿色荧光蛋白 转基因山羊乳腺上皮细胞 |
| 文章编号： | 0366-6964（2006）12-1282-05 |
| 收稿时间： | 2005-09-16 |
| 修稿时间： | 2005-09-16 |
Study on the hLF Gene Transgenic Goat Mammary Epithelial Cells |
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| ZHENG Yue-mao,LIU Feng-jun,HE Xiao-ning,ZHANG Yong. Study on the hLF Gene Transgenic Goat Mammary Epithelial Cells[J]. Chinese Journal of Animal and Veterinary Sciences, 2006, 37(12): 1282-1286 |
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| Authors: | ZHENG Yue-mao LIU Feng-jun HE Xiao-ning ZHANG Yong |
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| Abstract: | The recombinant plasmid containing human lactoferrin gene cDNA was transfected into goat mammary epithelial cells after liposome transinfection. Positive cells were selected by G418 and PCR. Positive cells were proliferated and induced by hormone. The result of Western blotting analysis on cultured cell supernatant showed that the transfected cells could express and secrete hLF protein, whose MW was 58 ku. |
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| Keywords: | human lactoferrin gene mammary gland-specific expressional vector EGFP transgenic goat mammary epithelial cells |
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