|
|||||
|
|
| 猪带绦虫六钩蚴重组蛋白TSOL18与BSA偶连蛋白的制备 | |
| 引用本文： | 张少华,李克生,景志忠,杜惠芬,骆学农,才学鹏. 猪带绦虫六钩蚴重组蛋白TSOL18与BSA偶连蛋白的制备[J]. 甘肃农业大学学报, 2007, 42(3): 7-11 |
| 作者姓名： | 张少华 李克生 景志忠 杜惠芬 骆学农 才学鹏 |
| 作者单位： | 1. 甘肃农业大学动物医学院,甘肃,兰州,730070;中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,甘肃省动物寄生虫病重点实验室,甘肃,兰州,730046 2. 甘肃省医学科学研究院医学生物技术中心,甘肃,兰州,730050 3. 中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,甘肃省动物寄生虫病重点实验室,甘肃,兰州,730046 |
| 基金项目： | 甘肃省科技型中小型企业技术创新基金 |
| 摘 要： | 应用异型双功能连接剂MBS(间-马来酰亚胺苯甲酸-N-羟基琥珀酰亚胺酯)将猪带绦虫六钩蚴18ku重组蛋白(TSOL18)和载体蛋白-牛血清白蛋白(BSA)进行连接,制备连接蛋白BSA-TSOL18,用以增强TSOL18的免疫原性.连接蛋白利用快速脱盐柱分离技术进行纯化;纯化后应用醋酸纤维薄膜电泳证明二者的结合情况;并用间接ELISA法检测不同摩尔比连接产物中抗原部分的活性.结果表明:TSOL18与BSA偶连摩尔比为1∶3时连接有效,且对抗原活性影响小,可满足进一步免疫的要求. |
| 关 键 词： | 猪带绦虫 MBS TSOL18 BSA 蛋白连接 |
| 文章编号： | 1003-4315（2007）03-0007-05 |
| 修稿时间： | 2006-04-05 |
Preparation of coupled protein of BSA and recombinant oncosphere antigen TSOL18 |
|
| ZHANG Shao-hua,LI Ke-sheng,JING Zhi-zhong,DU Hui-fen,LUO Xue-nong,CAI Xue-peng. Preparation of coupled protein of BSA and recombinant oncosphere antigen TSOL18[J]. Journal of Gansu Agricultural University, 2007, 42(3): 7-11 | |
| Authors: | ZHANG Shao-hua LI Ke-sheng JING Zhi-zhong DU Hui-fen LUO Xue-nong CAI Xue-peng |
| Affiliation: | 1. College of Veterinary Medieine,Gansu Agricultural University,Lanzhou 730070,China; 2. Key Laboratory of Veterinary Parasitology of Gansu Province,State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agrieuhural Sciences, Lanzhou 730046, China; 3. Research Center of Medical BioTeeh,Gansu Medical Research Institute,Lanzhou 730050,China |
| Abstract: | The recombinant oncosphere antigen TSOL18 was coupled with carrier protein BSA by chemical cross-linker MBS to prepare coupled protein TSOL18 and improve the immunogenicity of it.The product BSA-MBS-TSOL18 was eluted through desalting column to purify.The linking degree between TSOL18 and BSA were testified by cellulose acetate membrane electrophoresis.The activity of the antigen part in the coupled protein,which was coupled in different ratio,was identified by indirect ELISA.The results showed that the linkage between TSOL18 and BSA was efficient when the coupling ratio was 1∶3. |
| Keywords: | Taenia solium MBS TSOL18 BSA conjugation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |