大豆对菌核病室内抗性鉴定方法研究

实验室条件下，利用菌丝悬液、麦粒菌丝接种体、ＰＤＡ菌丝块等接种不同大豆材料的钵载幼苗、离土整株及离体茎叶。结果表明：除Ｖ３期离体叶接种方式外，其它几种接种鉴定方法均能明显地区分不同大豆材料的抗感水平，并与疫区田间自然感病鉴定的结果基本符合。ＰＤＡ菌丝块Ｖ３期离体茎接种和菌丝悬液Ｖ３期整株接种，简便易行，是适于大规模筛选大豆抗源的有效方法，菌丝滤液Ｖ３期整株浸根鉴定，更为直观方便，在确定稳定的标准对

STUDIES ON THE METHODS OF EVALUATING VARLETAL RESISTANCEOF SOYBEAN TO SCLEROTINIA ROT UNDER LABORATORY CONDITIONS

New screening methods for evaluating resistance (tolerance) of soybean cultivars to sclero-tinia rot were tested The results showed significant differences in suseeptibility among cultivars.Inoculating soybean seedlings detached seedlings or detached stems at V3 stage with wycelial sus-pension, wheat-seed culture or PDA cultural block were all effective to differentiate resistance ofsoybean cultivars, with the only exception of detached leaf method. The results had a better ac-cordence with the field test. Both the methods of inoculation with PDA mycelia block and myceliasuspension were more convinent and suitable for screening resistant resources on large scale inlaboratory. Method of soaking roots with cultural filtrate was simple and may obtain results di-rectly. It would become and effective method for screening resistant resources if CK varieties withstable resistance were found.