| 4种罗非鱼不同组织中HSP70基因对盐胁迫的响应 |
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| 引用本文： | 刘志刚,刘玉姣,卢迈新,朱华平,可小丽,高风英,曹建萌,王淼.4种罗非鱼不同组织中HSP70基因对盐胁迫的响应[J].上海海洋大学学报,2015,24(2):182-189. |
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| 作者姓名： | 刘志刚 刘玉姣 卢迈新 朱华平 可小丽 高风英 曹建萌 王淼 |
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| 作者单位： | 中国水产科学研究院珠江水产研究所,中国水产科学研究院珠江水产研究所；上海海洋大学,中国水产科学研究院珠江水产研究所,中国水产科学研究院珠江水产研究所,中国水产科学研究院珠江水产研究所,中国水产科学研究院珠江水产研究所,中国水产科学研究院珠江水产研究所,中国水产科学研究院珠江水产研究所 |
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| 基金项目： | 十二五国家科技支撑计划项目(No.2012BAD26B03-02)；现代农业产业技术体系专项资金资助(CARS-49)；广东省海洋渔业科技推广专项(No.A201101C02) |
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| 摘 要： | 为了从分子水平评估莫桑比克罗非鱼、荷那龙罗非鱼及其正交子代(莫桑比克罗非鱼♀×荷那龙罗非鱼)和反交♂子代(荷那龙罗非鱼♀×莫桑比克罗非鱼)的耐盐性能,利♂用RT-PCR方法研究了盐胁迫下这4种罗非鱼不同组织中(鳃、肾、垂体和肌肉)热休克蛋白70(HSP70)基因的表达规律。结果表明:在盐度为22条件胁迫下4种罗非鱼各组织中HSP70表达水平总体呈现先增加后降低的规律,24 h时达最大值,48 h时下降至初始水平;盐度提高至35后HSP70的表达无显著变化。但不同种罗非鱼及不同组织之间也存在差异,反交子代鳃中HSP70在整个盐胁迫过程中都未上调表达;在盐度为22条件胁迫下,正反交子代肾中HSP70基因表达量比父母本肾中HSP70更早达到峰值;反交子代垂体中HSP70表达量在0~48 h内持续缓慢增加,48 h时达最大值;莫桑比克罗非鱼肌肉中HSP70基因在盐度22条件胁迫下表达量迅速升高,且维持较高水平直至盐度35条件胁迫之后。结果表明:HSP70表达水平的高低与罗非鱼的耐盐能力和应激程度密切相关,其中盐胁迫下肾中HSP70的表达规律与以上4种罗非鱼的耐盐能力之间相关性较强,可以作为评价这4种罗非鱼耐盐能力的潜在分子指标。
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| 关 键 词： | 莫桑比克罗非鱼(Oreochromis mossambicus) 荷那龙罗非鱼(O.hornorum) 正交子代 反交子代 盐胁迫 HSP70基因 |
| 收稿时间： | 2014/8/22 0:00:00 |
| 修稿时间： | 2014/10/30 0:00:00 |
The HSP70 gene expression response of four strains of tilapia to salt stress in different tissues |
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| LIU Zhigang,LIU Yujiao,LU Maixin,ZHU Huaping,KE Xiaoli,GAO Fengying,CAO Jianmeng and WANG Miao.The HSP70 gene expression response of four strains of tilapia to salt stress in different tissues[J].Journal of Shanghai Ocean University,2015,24(2):182-189. |
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| Authors: | LIU Zhigang LIU Yujiao LU Maixin ZHU Huaping KE Xiaoli GAO Fengying CAO Jianmeng and WANG Miao |
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| Institution: | Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, Guangdong, China,Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, Guangdong, China;College of Fisheries & Life, Shanghai Ocean University, Shanghai 201306, China,Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, Guangdong, China,Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, Guangdong, China,Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, Guangdong, China,Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, Guangdong, China,Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, Guangdong, China and Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, Guangdong, China |
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| Abstract: | |
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| Keywords: | Oreochromis mossambicus Oreochromis hornorum cross hybrid reciprocal cross hybrid salt stress HSP70 gene |
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