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4种罗非鱼不同组织中HSP70基因对盐胁迫的响应
引用本文:刘志刚,刘玉姣,卢迈新,朱华平,可小丽,高风英,曹建萌,王淼.4种罗非鱼不同组织中HSP70基因对盐胁迫的响应[J].上海海洋大学学报,2015,24(2):182-189.
作者姓名:刘志刚  刘玉姣  卢迈新  朱华平  可小丽  高风英  曹建萌  王淼
作者单位:中国水产科学研究院珠江水产研究所,中国水产科学研究院珠江水产研究所;上海海洋大学,中国水产科学研究院珠江水产研究所,中国水产科学研究院珠江水产研究所,中国水产科学研究院珠江水产研究所,中国水产科学研究院珠江水产研究所,中国水产科学研究院珠江水产研究所,中国水产科学研究院珠江水产研究所
基金项目:十二五国家科技支撑计划项目(No.2012BAD26B03-02);现代农业产业技术体系专项资金资助(CARS-49);广东省海洋渔业科技推广专项(No.A201101C02)
摘    要:为了从分子水平评估莫桑比克罗非鱼、荷那龙罗非鱼及其正交子代(莫桑比克罗非鱼♀×荷那龙罗非鱼)和反交♂子代(荷那龙罗非鱼♀×莫桑比克罗非鱼)的耐盐性能,利♂用RT-PCR方法研究了盐胁迫下这4种罗非鱼不同组织中(鳃、肾、垂体和肌肉)热休克蛋白70(HSP70)基因的表达规律。结果表明:在盐度为22条件胁迫下4种罗非鱼各组织中HSP70表达水平总体呈现先增加后降低的规律,24 h时达最大值,48 h时下降至初始水平;盐度提高至35后HSP70的表达无显著变化。但不同种罗非鱼及不同组织之间也存在差异,反交子代鳃中HSP70在整个盐胁迫过程中都未上调表达;在盐度为22条件胁迫下,正反交子代肾中HSP70基因表达量比父母本肾中HSP70更早达到峰值;反交子代垂体中HSP70表达量在0~48 h内持续缓慢增加,48 h时达最大值;莫桑比克罗非鱼肌肉中HSP70基因在盐度22条件胁迫下表达量迅速升高,且维持较高水平直至盐度35条件胁迫之后。结果表明:HSP70表达水平的高低与罗非鱼的耐盐能力和应激程度密切相关,其中盐胁迫下肾中HSP70的表达规律与以上4种罗非鱼的耐盐能力之间相关性较强,可以作为评价这4种罗非鱼耐盐能力的潜在分子指标。

关 键 词:莫桑比克罗非鱼(Oreochromis  mossambicus)  荷那龙罗非鱼(O.hornorum)  正交子代  反交子代  盐胁迫  HSP70基因
收稿时间:2014/8/22 0:00:00
修稿时间:2014/10/30 0:00:00

The HSP70 gene expression response of four strains of tilapia to salt stress in different tissues
LIU Zhigang,LIU Yujiao,LU Maixin,ZHU Huaping,KE Xiaoli,GAO Fengying,CAO Jianmeng and WANG Miao.The HSP70 gene expression response of four strains of tilapia to salt stress in different tissues[J].Journal of Shanghai Ocean University,2015,24(2):182-189.
Authors:LIU Zhigang  LIU Yujiao  LU Maixin  ZHU Huaping  KE Xiaoli  GAO Fengying  CAO Jianmeng and WANG Miao
Institution:Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, Guangdong, China,Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, Guangdong, China;College of Fisheries & Life, Shanghai Ocean University, Shanghai 201306, China,Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, Guangdong, China,Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, Guangdong, China,Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, Guangdong, China,Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, Guangdong, China,Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, Guangdong, China and Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, Guangdong, China
Abstract:
Keywords:Oreochromis mossambicus  Oreochromis hornorum  cross hybrid  reciprocal cross hybrid  salt stress  HSP70 gene
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