Carbohydrate degrading enzyme production by plant pathogenic mycelia and microsclerotia isolates of Macrophomina phaseolina through koji fermentation |
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Authors: | Surinder Kaur G.S. DhillonS.K. Brar V.B. Chauhan |
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Affiliation: | a Department of Mycology & Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University (BHU), Varanasi 221005, India b INRS-ETE, Université du Québec, 490, Rue de la Couronne, Québec, Canada G1K 9A9 |
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Abstract: | The ability of hydrolytic enzyme production by two different isolates of Macrophomina phaseolina was studied on apple pomace as a substrate for solid state fermentation (SSF). Initial moisture level, temperature and fermentation period was optimized so as to achieve higher output. Among the two different isolates, microsclerotial (MphP) and mycelial (MphM), MphP was observed as a potential source of different hydrolytic enzymes as compared to MphM. MphP gave higher enzyme activities (IU/gram dry substrate (gds): filter paper cellulase (FPase) activity 196.21 ± 16.3 (120 h), carboxymethyl cellulase (CMCase) 279.34 ± 28.25 (72 h), β-glucosidase (BGL) 129.82 ± 12.41 (96 h), xylanase 2527.88 ± 46.15 (120 h), and amylase 2780.72 ± 38.13 (96 h), respectively at 70% (v/w) IML. The incubation temperature was also found to have impact on the enzyme production ability of Macrophomina strains. The higher enzyme activities were achieved (IU/gds) as follows FPase 276.13 ± 25.02 (40 °C, 120 h), CMCase 278.11 ± 24.47 (35 °C, 144 h), BGL 189.47 ± 15.05 (30 °C, 144 h), xylanase 3845.77 ± 43.38 (35 °C, 144 h) and amylase activity of 3309.45 ± 29.22 (35 °C, 120 h), respectively using MphP at 70% (v/w) IML. This study reports for the first time the potential of carbohydrate degrading enzyme bioproduction by different isolates of M. phaseolina. |
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Keywords: | ANOVA, analysis of variance AP, apple pomace BGL, β-glucosidase CWDEs, cell wall degrading enzymes CM, Case carboxymethyl cellulose DNS, dinitrosalicylic acid pNPG, p-nitrophenol egl, endoglucanase gene FPase, filter paper cellulase activity g/l, grams per litre h, hour IML, initial moisture level IU/gds, international unit/gram dry substrate MphM, Macrophomina phaseolina (mycelia) MphP, Macrophomina phaseolina (microsclerotia) SSF, solid state fermentation |
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