首页 | 本学科首页   官方微博 | 高级检索  
     


Chemical profiling and biological screening of Thymus lotocephalus extracts obtained by supercritical fluid extraction and hydrodistillation
Authors:Patrí  cia CostaSandra Gonç  alves,Clara GrossoPaula B. Andrade,Patrí  cia Valentã  oM. Gabriela Bernardo-Gil,Anabela Romano
Affiliation:a Institute for Biotechnology and Bioengineering, Centre of Genomics and Biotechnology (IBB-CGB), Faculty of Sciences and Technology, University of Algarve, Campus de Gambelas, Ed. 8, 8005-139 Faro, Portugal
b REQUIMTE/Laboratório de Farmacognosia, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, R. Aníbal Cunha, 164, 4050-047 Porto, Portugal
c Centre for Biological and Chemical Engineering, IBB; DEQB, IST, Av. Rovisco Pais, 1049-001 Lisboa, Portugal
Abstract:Essential oil and extracts from the aerial parts of Thymus lotocephalus were obtained by hydrodistillation (HD) and supercritical fluid extraction (SFE) in two different collectors, respectively. SFE was conducted at 40 °C and a working pressure of 12 or 18 MPa. The chemical profiles were determined using GC-FID and GC-IT-MS. Oxygen-containing monoterpenes were the primary constituents in the essential oil and SFE extracts collected in the second separator, while the extracts obtained in the first separator were predominantly oxygen-containing sesquiterpenes. A large number of compounds were identified by hydrodistillation and, in contrast, the highest extraction yields were obtained using SFE. Linalool (10.43 ± 1.63%) was the main component in essential oil, whereas camphor (7.91 ± 0.84%) and cis-linalool oxide (7.25 ± 1.45%) were the major compounds in the extracts-2nd separator obtained at pressures of 12 and 18 MPa, respectively. Caryophyllene oxide was the primary constituent identified in the extracts-1st separator (4.34 ± 0.51 and 4.41 ± 1.25% obtained at 12 and 18 MPa, respectively). The antioxidant activity was assessed by ORAC and DPPH assays, and the anti-cholinesterase activity was evaluated in vitro using Ellman's method. The essential oil and SFE extracts (first separator) of T. lotocephalus possessed antioxidant activity and strongly inhibited cholinesterases. We also demonstrated that the acetylcholinesterase and butyrylcholinesterase inhibitory activities of the essential oil could be attributed to 1,8-cineole and caryophyllene oxide, respectively.
Keywords:AAPH, 2,2&prime  -azobis(2-amidinopropane) dihydrochloride   ACh, acetylcholine   AChE, acetylcholinesterase   AD, Alzheimer's disease   ATCI, acetylthiocholine iodide   BChE, butyrylcholinesterase   BTCI, butyrylthiocholine chloride   ChE, cholinesterase   CO2, carbon dioxide   DTNB, 5,5&prime  -dithiobis[2-nitrobenzoic acid]   DPPH, 2,2-diphenyl-1-picrylhydrazyl   BHT, butylated hydroxytoluene   SET, single electron transfer   GC-FID, gas chromatography-flame ionisation detector   GC-IT-MS, gas chromatography-ion trap-mass spectrometry   HAT, hydrogen atom transfer   HD, hydrodistillation   ORAC, oxygen radical absorbance capacity   ROS, reactive oxygen species   SFE, supercritical fluid extraction   TE, trolox equivalents   Trolox, 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid
本文献已被 ScienceDirect 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号