Chitosan enhances withanolides production in adventitious root cultures of Withania somnifera (L.) Dunal |
| |
Authors: | G SivanandhanM Arun S MayavanM Rajesh TS MariashibuM Manickavasagam N SelvarajA Ganapathi |
| |
Institution: | a Department of Biotechnology, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India b Temasek Life Sciences Laboratory Limited, 1 Research Link, National University of Singapore, Singapore 117604, Singapore c Periyar E. V. R College (Autonomous), Tiruchirappalli 620 023, Tamil Nadu, India |
| |
Abstract: | Calli were obtained from leaf, cotyledon and internode explants of in vitro-grown plants of Indian cultivar of Withania somnifera in MS medium supplemented with 2, 4-D (2.0 mg l−1) and Kinetin (0.2 mg l−1). The brown, semi-friable callus (500 mg FW) derived from leaf explants produced higher number of primary adventitious roots (9 roots/callus) in half strength MS medium fortified with IBA (0.5 mg l−1) and NAA (0.1 mg l−1). The primary adventitious roots with an inoculum mass of 15 g FW were cultured for 6 weeks in the same medium for secondary adventitious root proliferation. Elicitation of abiotic elicitor, aluminium chloride at 10 mg l−1 at the end of 4 weeks culture with 4 h exposure time enhanced withanolides productivity. Under similar culture conditions, the biotic elicitor, chitosan at 100 mg l−1 stimulated higher production of all withanolides when compared to aluminium chloride treatment. This is the first report on the use of callus-derived adventitious root culture for the enhanced production of withanolides upon chitosan elicitation. |
| |
Keywords: | Adventitious root culture Aluminium chloride Chitosan Withania somnifera Withanolides |
本文献已被 ScienceDirect 等数据库收录! |
|