山梨醇对李果肉组织总RNA提取的影响

探讨了不同浓度的山梨醇清洗溶液对李果肉组织中RNA提取效果的影响。经琼脂糖凝胶电泳、核酸蛋白分析仪和RT-PCR等方法对RNA质量和产率进行了分析,结果表明:李果肉组织研磨后用山梨醇清洗溶液处理有利于总RNA的提取,而不添加山梨醇的清洗溶液,提取液中检测不到RNA,当清洗溶液中山梨醇浓度为1.10mol/L时,李果肉组织总RNA的产率明显高于山梨醇浓度为0.35 mol/L和3.00 mol/L时的产率;以不同贮藏天数的李果实为试材提取总RNA,其结果是一致的;通过RT-PCR扩增蛋白延伸因子EF-2基因,结果证明本试验提取的总RNA可以用于基因转录表达特性分析。

Effect of Sorbitol on Total RNA Isolation from Plum Fruit Flesh

The effects of sorbitol washing buffer on total RNA isolation from plum fruit flesh were investigated.The quality and yield of total RNA were tested by agarose gel electrophoresis,nucleic acid/protein analysis and RT-PCR assay.The results showed that sorbitol was helpful for total RNA isolation from plum fruit flesh.When the sorbitol concentration in sorbitol buffer was 1.10 mol/L,the yield of total RNA was obviously higher,but the total RNA couldn't be detected without sorbitol in washing buffer.The result...