| 两株芽孢杆菌的鉴定及淀粉酶基因的克隆 |
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| 引用本文： | 胡苗清,姚明泽,张耀华,付月君,梁爱华.两株芽孢杆菌的鉴定及淀粉酶基因的克隆[J].华北农学报,2011(5):103-106. |
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| 作者姓名： | 胡苗清 姚明泽 张耀华 付月君 梁爱华 |
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| 作者单位： | 山西大学生物工程教育部重点实验室生物技术研究所; |
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| 基金项目： | 国家自然科学基金项目(31071924); 山西省自然科学基金项目(2009011059-3;2010011040-1) |
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| 摘 要： | 对分离得到的两个菌株(B.H和B.M)进行鉴定,并对它们在芽孢杆菌培养基和淀粉富集培养基上的形态进行观察,通过对其16S rDNA序列的分析,构建系统进化树,确定B.H为枯草芽孢杆菌,B.M为解淀粉芽孢杆菌.根据已报道的枯草芽孢杆菌和解淀粉芽孢杆菌淀粉酶基因保守区域设计引物,分别以两个菌株的基因组DNA为模板,采用PC...
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| 关 键 词： | 枯草芽孢杆菌 解淀粉芽孢杆菌 16S rDNA 淀粉酶基因 |
Identification of Two Species of Bacillusand Gene Cloning of Amylase |
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| HU Miao-qing,YAO Ming-ze,ZHANG Yao-hua,FU Yue-jun,LIANG Ai-hua.Identification of Two Species of Bacillusand Gene Cloning of Amylase[J].Acta Agriculturae Boreali-Sinica,2011(5):103-106. |
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| Authors: | HU Miao-qing YAO Ming-ze ZHANG Yao-hua FU Yue-jun LIANG Ai-hua |
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| Institution: | HU Miao-qing,YAO Ming-ze,ZHANG Yao-hua,FU Yue-jun,LIANG Ai-hua(Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education,Institute of Biotechnology,Shanxi University,Taiyuan 030006,China) |
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| Abstract: | Two isolated strains of Bacillus(B.H and B.M)were identified.Morphologic observation on the Bacillus medium and the starch enrichment medium,and the analysis of the 16S rDNA sequences and Phylogenetic Trees showed that B.H is Bacillus subtilis and B.M is Bacillus amyloliquefaciens.The primes were designed according to conservative sequences of amylase of Bacillus,using each genomic DNA of two strains as templates.The lengths of coding area are 1 434 bp and 1 563 bp respectively.These genes provide the exper... |
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| Keywords: | Bacillus subtilis Bacillus amyloliquefaciens 16S rDNA Amylase gene |
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