| 甘蓝型油菜小孢子培养影响因素研究及再生苗早期倍性鉴定 |
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| 引用本文： | 米哲,李云昌,梅德圣,李英德,徐育松,陈玉峰,胡琼.甘蓝型油菜小孢子培养影响因素研究及再生苗早期倍性鉴定[J].华北农学报,2011(5):97-102. |
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| 作者姓名： | 米哲 李云昌 梅德圣 李英德 徐育松 陈玉峰 胡琼 |
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| 作者单位： | 中国农业科学院油料作物研究所国家油料作物改良中心农业部油料作物生物学重点开放实验室; |
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| 基金项目： | 国家科技支撑计划(2010BAD35B04); 油菜现代产业技术体系建设(ngcytx-005) |
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| 摘 要： | 对不同年份、不同地点种植的油菜育种材料进行小孢子产胚率鉴定,并以11种不同基因型的甘蓝型油菜品系为供体材料,在武汉田间,分别于初花期和盛花期分离培养小孢子,研究基因型、生态条件、开花时间对小孢子产胚率的影响及秋水仙碱处理的加倍效果,通过采用流式细胞仪测定小孢子再生苗的DNA含量鉴定其倍性,并种植验证.结果表明:相同基因...
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| 关 键 词： | 油菜 小孢子培养 DNA含量 倍性鉴定 |
Factors Affecting Microspore Embryogenesis and Early Ploidy Level Determination of Regenerated Plants in Brassica napus |
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| MI Zhe,LI Yun-chang,MEI De-sheng,LI Ying-de,XU Yu-song,CHEN Yu-feng,HU Qiong.Factors Affecting Microspore Embryogenesis and Early Ploidy Level Determination of Regenerated Plants in Brassica napus[J].Acta Agriculturae Boreali-Sinica,2011(5):97-102. |
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| Authors: | MI Zhe LI Yun-chang MEI De-sheng LI Ying-de XU Yu-song CHEN Yu-feng HU Qiong |
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| Institution: | MI Zhe,LI Yun-chang,MEI De-sheng,LI Ying-de,XU Yu-song,CHEN Yu-feng,HU Qiong(Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,National Center for Oil Crops Improvement,Key Laboratory for Biological Sciences of Oil Crops,Ministry of Agriculture,Wuhan 430062,China) |
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| Abstract: | The breeding materials of Brassica napus grown in different ecological regions and years were used to determine their microspore embryogenesis capacity.The microspores of 11 genotypes grown in field condition of Wuhan,Hubei Province,were isolated at different flowering stages and cultured.The aim of this study was to investigate the effects of genotype,ecological condition and sampling time on microspore embryogenesis,as well as the colchicine treatment on chromosome doubling of regenerated seedlings.Ploidy... |
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| Keywords: | Oilseed rape Microspore culture DNA content Ploidy identification |
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