| 槲皮素和硫脲对野桑蚕多酚氧化酶的抑制类型 |
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| 引用本文： | 张永亮,朱勇.槲皮素和硫脲对野桑蚕多酚氧化酶的抑制类型[J].湖北农业科学,2011(21):4399-4400. |
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| 作者姓名： | 张永亮 朱勇 |
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| 作者单位： | 周口师范学院生命科学系;西南大学生物技术学院; |
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| 基金项目： | 教育部博士学科点专项基金项目(20060635008); 重庆市教委基金项目(040208) |
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| 摘 要： | 多酚氧化酶(Polyphenol oxidase,PPO)在昆虫的变态发育和免疫防御中起着非常重要的作用,为开发以该酶为靶标的新型害虫抑制剂提供理论依据,以野桑蚕(Bombyx mandarina)五龄幼虫为材料提取多酚氧化酶,探讨了槲皮素和硫脲对该酶的抑制作用。结果表明,这两种抑制剂分别属于竞争性和非竞争性抑制剂。
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| 关 键 词： | 野桑蚕(Bombyx mandarina) 多酚氧化酶 槲皮素 硫脲 抑制类型 |
Inhibitory Types of Quercetin and Thiourea on the Polyphenoloxidase from Bombyx mandarina |
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| ZHANG Yong-liang,ZHU Yong.Inhibitory Types of Quercetin and Thiourea on the Polyphenoloxidase from Bombyx mandarina[J].Hubei Agricultural Sciences,2011(21):4399-4400. |
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| Authors: | ZHANG Yong-liang ZHU Yong |
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| Institution: | ZHANG Yong-liang1,ZHU Yong2 (1.Department of Life Sciences,Zhoukou Normal University,Zhoukou 466001,Henan,China,2.School of Biotechnology,Southwest University,Chongqing 400715,China) |
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| Abstract: | Polyphenol oxidase plays a very important role in the immune defense and metamorphosis development of insect.In order to provide the theoretical basis for studying new pest inhibitor targeting at the enzyme,the inhibition effects of quercetin and thiourea on polyphenol oxidase from Bombyx mandarina was approached.The results indicated that quercetin and thiourea was competitive and non-competitive inhibitor,respectively. |
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| Keywords: | Bombyx mandarina polyphenol oxidase quercetin thiourea inhibitory types |
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