| 亚麻显性核不育相关基因的克隆及序列分析 |
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| 引用本文： | 高凤云,张辉,贾霄云,斯钦巴特尔.亚麻显性核不育相关基因的克隆及序列分析[J].华北农学报,2011(5):54-57. |
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| 作者姓名： | 高凤云 张辉 贾霄云 斯钦巴特尔 |
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| 作者单位： | 内蒙古农牧业科学院; |
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| 基金项目： | 国家自然基金(30460069); 内蒙古自然基金(200508010301); 胡麻产业技术体系(nycytx-22) |
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| 摘 要： | 亚麻显性雄性核不育基因对亚麻育种以及杂种优势的利用具有相当重要的作用,为更好地利用这一优良生物性状,很有必要对其相关基因进行标记、克隆和序列分析.通过使用252条10-mer的随机引物对遗传背景相似的兄妹杂交分离出的17对可育株和不育株亚麻进行RAPD分子标记,得到三条差异片段,通过回收、克隆及测序,然后用BLAST进...
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| 关 键 词： | 亚麻 显性核不育 克隆 序列分析 |
Gene Clone and Sequence Analysis of Flax Associated with Dominant Male Sterile |
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| GAO Feng-yun,ZHANG Hui,JIA Xiao-yun,SI Qin-ba-teer.Gene Clone and Sequence Analysis of Flax Associated with Dominant Male Sterile[J].Acta Agriculturae Boreali-Sinica,2011(5):54-57. |
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| Authors: | GAO Feng-yun ZHANG Hui JIA Xiao-yun SI Qin-ba-teer |
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| Institution: | GAO Feng-yun,ZHANG Hui,JIA Xiao-yun,SI Qin-ba-teer(Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences,Huhhot 010031,China) |
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| Abstract: | Dominant male sterile gene of flax is very important for flax breeding and using of heterosis.For better utilization of these genes,It is necessary to marker and clone and sequence these genes.Three specific DNA fragments were gained by RAPD marker with 252 arbitrary 10-mer primers to identify dominant sterile gene of 17 pair of sterile and fertile flaxes breeding from parents with the same genetic background.Clone and Sequence as well as comparison of the fragments showed that the fragments is homology to ... |
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| Keywords: | Flax Dominant sterile gene Clone Sequence analysis |
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