| 用响应面分析法优化仙人掌多糖提取工艺参数 |
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| 引用本文： | 高宛莉,柴春月,杜瑞卿. 用响应面分析法优化仙人掌多糖提取工艺参数[J]. 安徽农业科学, 2007, 35(23): 7090-7091 |
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| 作者姓名： | 高宛莉 柴春月 杜瑞卿 |
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| 作者单位： | 河南南阳师范学院生命科学系,河南南阳,473061;河南南阳师范学院生命科学系,河南南阳,473061;河南南阳师范学院生命科学系,河南南阳,473061 |
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| 摘 要： | 选取仙人掌多糖提取时间、提取温度和料液比3个因素进行二次回归正交组合设计试验,利用响应面法对其提取工艺参数进行优化。结果表明:在提取时间为132 min、温度为74℃、水体积为276 ml的条件下,仙人掌多糖提取产量最高,最大提取产量预测值为89.77μg/ml,实际提取值89.48μg/ml,二者基本相符。利用优化工艺参数提取仙人掌多糖时,具有最大的提取产量。
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| 关 键 词： | 仙人掌 多糖 响应面法 提取工艺参数 |
| 文章编号： | 0517-6611（2007）23-07090-02 |
| 修稿时间： | 2007-04-13 |
Optimization of Extraction Technique of Opuntia Polysaccharide via Response Surface Methodology |
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| GAO Wan-li et al. Optimization of Extraction Technique of Opuntia Polysaccharide via Response Surface Methodology[J]. Journal of Anhui Agricultural Sciences, 2007, 35(23): 7090-7091 |
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| Authors: | GAO Wan-li et al |
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| Affiliation: | Department of Biology, Nanyang Normal University, Nanyang, Henan 473061 |
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| Abstract: | Effects of different temperature,time and the ratio of solid to solution on the extraction yield of polysaccharide from Opuntia were investigated based on composite design of quadratic regression.Extraction technique parameters were optimized with Response Surface Methodology.Experimental data were analyzed by solving the regression equation with MATLAB 7.0 software.It was indicated that the optimum extraction parameters were 74 ℃,132 min and 276ml water volume.Under those conditions,the predicted value of polysaccharide extraction yield from Opuntia was 89.77 μg/ml,which was in consistent with the measured value of 89.48 μg/ml.This method with optimized technique parameters had maximal extraction yield of polysaccharide from Opuntia. |
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| Keywords: | Opuntia Polysaccharide Response surface methodology Extraction technique |
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