Molecular characterization of Desmodium species — An important ingredient of ‘Dashmoola’ by RAPD analysis |
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Authors: | Saba Irshad Jyotsna Singh Poonam Kakkar Shanta Mehrotra |
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Affiliation: | 1. Emeritus Scientist Project, National Botanical Research Institute, Lucknow, 226001, India;2. Herbal Research Section, Indian Institute of Toxicology Research, Post Box No. 80, Mahatma Gandhi Marg, Lucknow, 226001, India |
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Abstract: | Identification of medicinal plants by their molecular signature is a fast growing tool. The identification of Desmodium gangeticum (L.) DC. (Shalparni, a constituent of Ayurvedic formulation “Dashmoolarishtha”) was carried out using genomic approach. Authentic samples of D. gangeticum(L.) DC., D. velutinum (Willd.) DC. and D. triflorum (L.) DC. were analyzed and compared to commercial samples of various origin. Within twenty primers used, eleven gave 223 RAPD fragments. RAPD profiles of three species showed very low similarity index (0.21–0.39), whereas market samples showed high similarity of 0.82–0.89 with authenticated D. gangeticum. |
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Keywords: | Desmodium gangeticum D. velutinum D. triflorum Dashmoola RAPD Medicinal plants |
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