| 一株嗜热纤维素分解菌液体发酵条件初步研究 |
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| 引用本文： | 张辉,朱奇,朱蓝英.一株嗜热纤维素分解菌液体发酵条件初步研究[J].天津农业科学,2006,12(1):15-17. |
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| 作者姓名： | 张辉 朱奇 朱蓝英 |
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| 作者单位： | 聊城大学生命科学学院,山东,聊城,252059 |
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| 摘 要： | 对嗜热侧孢霉(Thermophilic sporotrichum)液体发酵产纤维素酶的条件进行了初步研究,单因子实验结果表明,碳源为甘蔗渣+稻草粉时,CM Case与FPAase均最高,最适氮源是蛋白胨,最适pH为5.5,最适生长温度为45℃,培养第2天FPAase最高,培养第5天CMCase最高。正交试验结果表明,碳源(含量为1.5%)最佳配比是1∶4(稻草粉:甘蔗渣),蛋白胨的最佳含量为0.4%,KH2PO4最佳含量为0.3%,MgSO4.7H2O最佳含量为0.03%,最适pH为5.5,在最佳条件下培养时,测得CMCase为34.32IU,FPAase为9.44IU。
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| 关 键 词： | 纤维素 嗜热侧孢霉 液体发酵 |
| 文章编号： | 1006-6500（2006）01-0015-03 |
| 收稿时间： | 2006-01-10 |
| 修稿时间： | 2006-02-21 |
Study on the Optimum Conditions for Liquid Fermentation of a Thermophilic Microorganism to Produce Cellulase |
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| ZHANG Hui,ZHU Qi,ZHU Lan-ying.Study on the Optimum Conditions for Liquid Fermentation of a Thermophilic Microorganism to Produce Cellulase[J].Tianjin Agricultural Sciences,2006,12(1):15-17. |
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| Authors: | ZHANG Hui ZHU Qi ZHU Lan-ying |
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| Institution: | College of Life Science, Liaocheng University, Liaocheng, Shandong 252059, China |
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| Abstract: | The study focused on the optimum conditions for liquid fermentation of Thermophilic sporotrichum.It was reported that the optimum carbon source is bagasse+straw powder(total content is 1.5% with the proportion of 1∶4),the appropriate nitrogen source is peptone(with 0.4% total content),while the optimum medium pH is 5.5 and 45 ℃ as culturing temperature.The optimum content of KH2PO4,MgSO4·7H2O is 0.3% and 0.03%,respectively,pH equals 5.5.When cultured under these conditions,CMCase is 34.32IU and FPAase is 9.44IU. |
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| Keywords: | cellulose Thermophilic sporotrichum liquid fermentation |
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