M1TDS技术及镉低积累杂交水稻亲本创制与组合选育

"镉大米"问题是当下中国南方水稻产业发展急需解决的重大问题,培育镉低积累水稻品种是解决该问题最直接、最经济的方法.本研究开发了一项高通量靶向鉴定理化诱变M1代突变及获取突变体的技术(M1 generation targeted deep sequencing technology,M1TDS技术).利用M1TDS技术,...

M;TDS Technology and Creation of Low-cadmium Accumulation Parents for Hybrid Rice Breeding

The problem of"cadmium-contaminated rice"is an urgent issue for the development of rice industry in south China.Breeding rice cultivars with low-cadmium accumulation in grains is the most direct and economical way to solve this problem.Here,we developed a technology of high-throughput screening for chimeric mutants in M₁ generation based on targeted deep sequencing,so-called as M₁ generation targeted deep sequencing(M₁TDS)technology.Using this technology,chimeric rice plants with mutations of cadmium transporter gene OsNramp5 were screened out from the heavy ion mutagenized plants in M₁ generation,and the sterile line Lian 1 S and restorer line R001 carrying homozygous osnramp5 gene were obtained in M2 generation.Furthermore,in the fields contaminated with an effective soil cadmium content of 0.443 mg/kg at pH 4.93,the grain cadmium content of the hybrid rice Lianliangyou 1 derived from Lian 1 S and R001 was stable below 0.03 mg/kg,much lower than that of control hybrid rice Zhenliangyou 8612(0.38 mg/kg)and the national limit standard of 0.20 mg/kg.This study not only provides important new germplasms for solving the problem of"cadmium-contaminated rice",but breaks the technical bottleneck of high-throughput identification of chimeric mutants in the M₁ generation of physicochemical mutagenesis,so as to provide a generic technology for targeted improvement of germplasms in physicochemical mutagenesis for rice and other crops.