| 枣胚培技术体系的建立 |
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| 引用本文： | 杜学梅,李登科,王永康,隋串玲,樊保国. 枣胚培技术体系的建立[J]. 园艺学报, 2005, 32(3): 496-499 |
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| 作者姓名： | 杜学梅 李登科 王永康 隋串玲 樊保国 |
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| 作者单位： | (山西省农业科学院果树研究所, 太谷 030815; 山西师范大学, 临汾 041000) |
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| 基金项目： | 山西省科技厅攻关项目(011023)，山西省资助回国留学人员科技项目(200383) |
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| 摘 要： | 以‘六月鲜’枣为试材, 对花后12～82 d的幼胚进行培养。结果表明, 适宜于胚挽救的最小胚龄为花后50 d左右; 在胚形成前进行离体培养, 蔗糖浓度以7%为宜, 而胚形成后离体培养以5%蔗糖为宜; 暗培养可促进离体胚的生长发育, 且有利于生根; 植物生长调节剂及有机添加物极显著地影响着胚培效果。花后70～72 d胚龄的胚适宜培养基为1 /2MS + IBA 0.4 (mg/L, 单位下同) +BA 0.8 +LH 500 + GA35 + 5%蔗糖。胚培苗经扩繁后转入1 /2 MS + IBA 1.0 + IAA 0.06 + 2%蔗糖培养基上, 获得了健壮生根苗,生根苗移栽成活率达85%以上。
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| 关 键 词： | 枣 胚 组织培养 |
| 文章编号： | 0513-353X(2005)03-0496-04 |
| 收稿时间： | 2004-08-12 |
| 修稿时间： | 2005-01-11 |
Estabilishnent of Embryo Culture Technical System of Chinese Jujube(Zizyphus jujuba Mill.) |
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| DU Xuemei,Li Dengke,WANG Yongkang,Sui Chuanling,Fan Baoguo. Estabilishnent of Embryo Culture Technical System of Chinese Jujube(Zizyphus jujuba Mill.)[J]. Acta Horticulturae Sinica, 2005, 32(3): 496-499 |
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| Authors: | DU Xuemei Li Dengke WANG Yongkang Sui Chuanling Fan Baoguo |
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| Affiliation: | ( Institute of Pomology, Shanxi Academy of Agricultural Sciences, Taigu 030815, China; Shanxi Normal Unirersity, Linfen 041000, China) |
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| Abstract: | The factors affecting embryo culture were studied in Zizyphus jujuba ‘Liuyuexian’. The youngest embryo saved successfullywas 50 days old. For the forming embryos, the op timal concentration of sucrose was 7% , for the formed ones was 5%. Dark treatment is beneficial to embryo sprouting, growing and rooting. There are extremely significance effects of plant growth regulators and organic substance on embryo culture. The media of 1 /2MS + IBA 0.4 (mg/L, the same below ) +BA 0.8 +LH 500 + GA3 5 + 5%sucrose is suitable for culture of 70 - 72 days old embryo. The rootless seedling should be transferred to 1 /2 MS + IBA 1.0 + IAA 0.06 + 2% sucrose to induce normal root system. |
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| Keywords: | Chinese jujube Embryo Tissue culture |
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