| 叶底红叶片外植体消毒方法的筛选 |
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| 引用本文： | 蔡坤秀,陈振东,林秀香,苏金强,余智城.叶底红叶片外植体消毒方法的筛选[J].热带农业科学,2010,30(1):1-2,5. |
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| 作者姓名： | 蔡坤秀 陈振东 林秀香 苏金强 余智城 |
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| 作者单位： | 福建省热带作物科学研究所,福建漳州,363001 |
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| 基金项目： | 福建省科技厅重点攻关项目 |
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| 摘 要： | 运用正交设计，对叶底红叶片外植物体进行不同消毒方法的灭菌效果比较和愈伤组织的诱导研究。结果表明，其最佳灭菌措施为：自来水冲洗60min、0．1％升汞浸泡25min、次氯酸钠20min。
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| 关 键 词： | 叶底红 消毒方法 组织培养 |
Screening of Appropriate Disinfection Method for Early Cultured Phyllagathis fordii Leaf Explant Tissue Culture |
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| CAI Kunxiu CHEN Zhendong LIN Xiuxiang SU Jinqiang YU Zhicheng.Screening of Appropriate Disinfection Method for Early Cultured Phyllagathis fordii Leaf Explant Tissue Culture[J].Chinese Journal of Tropical Agriculture,2010,30(1):1-2,5. |
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| Authors: | CAI Kunxiu CHEN Zhendong LIN Xiuxiang SU Jinqiang YU Zhicheng |
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| Institution: | CAI Kunxiu CHEN Zhendong LIN Xiuxiang SU Jinqiang YU Zhicheng(Fujian Institute of Tropical Crops,Zhangzhou,Fujian 363001) |
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| Abstract: | With leaves of Phyllagathis fordii as explants,orthogonal design method was used to chose the appropriate disinfection method for and study callus induction of early cultured Phyllagathis fordii tissue culture.The results indicated that the best disinfection method was rinsing the explants with tap water for 60 min,then immersing in 0.1% HgC12 for 25 min,and finally immersing in 2.0% sodium hypochlorite solution for 20 min. |
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| Keywords: | Phyllagathis fordii tissue culture disinfection method screening |
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