| 3种石斛兰种子非共生萌发和离体保存 |
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| 引用本文： | 余乐,兰芹英,王晓静,马双敏,梁国鲁.3种石斛兰种子非共生萌发和离体保存[J].安徽农学通报,2009,15(8):27-28. |
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| 作者姓名： | 余乐 兰芹英 王晓静 马双敏 梁国鲁 |
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| 作者单位： | 西南大学园艺园林学院,重庆,北碚,400715;中国科学院西双版纳热带植物园;云南大学生命科学学院 |
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| 基金项目： | 国家自然科学基金，中国科学院战略生物资源技术支撑体系专项项目，科学技术部平台项目 |
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| 摘 要： | 以金钗石斛（Dendrobium nobile）、流苏石斛（D.fimbriatum）和报春石斛（D.primulinum）为研究对象，研究了其在激素浓度配比下种子萌发情况，并对流苏石斛幼苗进行离体保存。结果表明：25℃时，Knudson C（KC）＋BA2mg／L＋N从0．2mg／L最利于金钗和流苏石斛原球茎诱导和幼苗分化；KC＋NAA0．5mg／L时最适报春石斛原球茎诱导和幼苗分化。幼苗保存在15℃，每天12h光照（20001x），添加3％甘露醇（mannitol）的KC培养基中，10个月后存活率为96％。
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| 关 键 词： | 石斛属 种子 非共生萌发 离体保存 |
Non-symbiotic Germination of Three Species in Dendrobium and In vitro Conservation at Low Temperature |
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| Yu Le,Lan Qingying,Wang Xiaojing,Ma Shuangmin,Liang Guolu.Non-symbiotic Germination of Three Species in Dendrobium and In vitro Conservation at Low Temperature[J].Auhui Agricultural Science Bulletin,2009,15(8):27-28. |
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| Authors: | Yu Le Lan Qingying Wang Xiaojing Ma Shuangmin Liang Guolu |
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| Institution: | Yu Le Lan Qingying Wang Xiaojing Ma Shuangmin Liang Guolu (1College of Horticulture and Landscape, Southwest University, Beibei, Chongqing 400715,China; 2Xishuangbanna Tropical Botanical Garden, The Chinese Academy of Sciences; 3Schoolof Life Sciences, Yunnan University ) |
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| Abstract: | Effect of different hormones combinations on the seed non-symbiotic germination of D. nobile, D. fimbriatum and D. primulinum also the in vitro conservation of D. fimbriatum seedings were studied in this paper. The results showed that KC＋ BA2mg/L ＋NAA0.2mg/Lat 25℃ was the best for D. nobile and D.fimbriatum protcorm growth and organogenesis; KC＋ NAA0.5mg/L at 25 ℃ was suitable for D. primulinum. Addition of mannitol （ 3.0%w/v ） to the medium and incubation at low temperature （ 15 ℃ ） resulted in higher survival rate （ 96% ） after 10 months of continuous storage. |
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| Keywords: | Dendrobium Seed Non-symbiotic Germination In vitro conservation |
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