转Bt基因棉不同品种杀虫蛋白季节性表达及其对棉铃虫的控制作用

实验室条件下研究了不同品种转Bt基因棉花对棉铃虫抗性的差异、不同生育期棉花抗虫性的变化及Bt毒蛋白季节性表达。结果显示,不同品种转Bt基因棉在不同发育阶段,其棉叶均有阻止棉铃虫取食的作用,表现了明显的抗性,但其抗性强弱存在明显差异。DP 99B抗性表现最好,Hanza154抗性相对较差,6 d平均校正死亡率分别为89.23%和75.91%。转基因棉不同品种对棉铃虫的抗性在时间上呈现动态变化,以植株发育阶段划分,表现为蕾期盛花期花铃期铃期。ELISA测定结果表明,Bt毒蛋白在转基因棉不同器官中的含量差异显著,叶片中Bt毒蛋白的表达量显著大于其它组织器官。随着棉花生长发育的推进,叶片(鲜重)中Bt毒蛋白含量则明显降低,表现为七叶期三叶期蕾期铃期花铃期。

Seasonal Expression of Bacillus thuringiensis Insecticidal Protein and the Control to Cotton Bollworm in Different Varieties of Transgenic Cotton

The difference for resistant effects to bollworm, the dynamic of resistance and the content of Bt toxin protein in different organs during developmental stages of different varieties of Bt transgenic cotton were explored in laboratory. The results showed that there were significant differences for resistance to cotton bollworm during every growing stage of all varieties; DP 99B has the highest resistance to cotton bollworm, while Hanza 154 has the lowest, and their average corrected mortality to cotton bollworm were 89.23% and 75.91%, respectively. The resistance to cotton bollworm of cotton plants in each growing stage was presented in the following order, budding stage > flowering stage > flowering-boll stage >boll stage. The results of ELISA showed that Bt toxin protein could be detected in all organs of transgenic cotton, but its content varied significantly among organs along the developmental stages. There were significant differences of Bt toxin protein content among the functional leaves in different stages, the order of the content was: seven-leave stage > three-leave stage > budding stage > boll stage > flowering-boll stage.