| 香蕉ACC氧化酶基因(MAO3)的克隆及其表达特性分析 |
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| 引用本文： | 黄俊生,王华,张世清. 香蕉ACC氧化酶基因(MAO3)的克隆及其表达特性分析[J]. 园艺学报, 2005, 32(5): 807-811 |
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| 作者姓名： | 黄俊生 王华 张世清 |
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| 作者单位： | 1. 中国热带农业科学院环境与植物保护研究所,儋州,571737
2. 中国热带农业科学院热带生物技术研究所热带作物生物技术国家重点实验室,海口,571101 |
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| 基金项目： | 国家自然科学基金资助项目(39560056) |
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| 摘 要： | 根据同源扩增得到香蕉(Musa acuminata) ACC氧化酶基因(MAO3) 的核心部分, 再通过3'和5'RACE扩增上、下游序列以及Genome-Walker的方法得到启动子部分, 共获得3 718 bp长度的序列。将所得结果进行聚类分析, 发现香蕉中ACC氧化酶的氨基酸序列非常保守, 各序列间的同一性高达99% ,与单子叶植物和双子叶植物中ACC氧化酶的氨基酸序列的同源性在66.7%～71.8%之间。组织原位杂交试验表明MAO3基因的表达具有组织特异性, 初步认为是在韧皮部筛管组织中特异表达。运用实时荧光定量PCR技术, 实时监测到了MAO3基因和香蕉乙烯受体基因ERS2受机械伤诱导的定量变化。
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| 关 键 词： | 香蕉 MAO3基因 克隆 同源性分析 组织特异表达 机械伤诱导 |
| 文章编号： | 0513-353X（2005）05-0807-05 |
| 收稿时间： | 2004-12-01 |
| 修稿时间： | 2004-12-012005-02-02 |
Cloning and Expression Analysis of Banana ACC Oxidase Gene(MAO3) |
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| Huang Junsheng,Wang Hua,Zhang Shiqing. Cloning and Expression Analysis of Banana ACC Oxidase Gene(MAO3)[J]. Acta Horticulturae Sinica, 2005, 32(5): 807-811 |
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| Authors: | Huang Junsheng Wang Hua Zhang Shiqing |
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| Affiliation: | 1 Enviroment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China; 2State Key Laboratory of Tropical Crop Biotechnology, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China |
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| Abstract: | We obtained core sequence of MAO3 of banana according to homo-cloning,and then cloned 5' up stream and 3' down stream sequences by RACE PCR technology and the promoter sequence by Genome-Walker technology. Phylogenetic analysis showed that this gene was highly conserved in the same species and had comparatively high homology compared with both monocots and dicots. In situ hybridization experiment showed that the expression of MAO3 gene was tissue specific. Meanwhile, according to fluorescence real-time quantification PCR, we found that MAO3 and ERS2(one of banana ethylene receptor genes)genes were also induced by wounding. |
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| Keywords: | Banana MAO3 gene Cloning Homology analysis Tissue specific expression analysis Wounding induce |
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