Relationships Among <Emphasis Type="Italic">Brassica napus</Emphasis> (L.) Germplasm from Spain and Great Britain as Determined by RAPD Markers |
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Authors: | Email author" target="_blank">María?Elena?CarteaEmail author Pilar?Soengas Ana?Picoaga Amando?Ordás |
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Institution: | (1) Misión Biológica de Galicia, Consejo Superior de Investigaciones Científicas (CSIC), Apartado 28, E-36080 Pontevedra, Spain |
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Abstract: | The genetic diversity and the relationships among a collection of Brassica napus L. European populations were evaluated using random amplified polymorphic DNA markers. The study included 33 accessions of
B. napus collected from Galicia (northwestern Spain) and 18 British cultivars, 16 accessions of B. napus and two accessions of Brassica oleracea L. used as controls. DNA from 25 individuals per population was analyzed using 18 decamer primers. One hundred thirty-eight
amplification products were scored of which 105 were polymorphic. These bands ranged in size from 350 to 2500 base pairs.
Similarity coefficients and cluster analysis were computed and six groups were obtained. Cluster I was the largest and included
all the landraces from northwestern Spain, except two accessions that grouped separately into Clusters III and IV, respectively.
A low level of genetic variability was detected among the B. napus Spanish genotypes, while considerable diversity was present among the British ones, which grouped into three groups, two
main clusters and one group formed by one accession. Cluster II included all commercial varieties grown in Great Britain whereas
Cluster V grouped local varieties maintained by the growers for many years. Cluster VI was a singularity formed by one entry.
British accessions of B. oleracea had the greatest dissimilarity with all the other populations and grouped separately in Clusters VII and VIII. As conclusion,
B. napus landraces used in northwestern Spain as leafy-green vegetable probably have an independent origin from B. napus crops grown in other European regions. Besides, separate domestication in northwestern Spain and Great Britain for a different
end use might have led to two distinct gene pools. |
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Keywords: | Brassica napus Genetic diversity Molecular markers Numerical taxonomy |
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