小麦幼胚愈伤组织培养和抗赤霉病体细胞筛选

IMMATURE EMBRYO CALLUS CULTURE AND SOMATIC SELECTION TO SCAB RESISTANCE IN WHEAT

In this experiment immature embryo callus culture technique was improved and used, and the observation of parents and its hybrid with scab crude toxic processing and nonprocessing was made to study the growth and development of the callus.The results showed., (1) In different genotypes, the potency and the frequency of callus formation, callus sizes and variance, the totipotent callus percentage, brown callus percentage,discarded callus percentage, the regeneration percentage were completely different. F_1 hybrid was prior to to the better parent and excel at parent means, which heterosis.(2)processed by scab crude toxic fluid, the callus growed inhibited, number of brown calli increase and regeneration percentage decrease.For recovery and regeneration after callus leaved toxic circumstance, F_1 hybrid was prior to both parents and show stronger toxic tolerant ability. (3)selected hybrids and improved varieties as mutant materials would more possibly result in successful somatic selection at cell level.