99份国内小麦新品种（系）醇溶蛋白的遗传多样性分析

为了解目前普通小麦育成品种（系）间醇溶蛋白的遗传多样性，利用酸性聚丙烯酰胺凝胶电泳（Acid polyacrylamide gel electrophoresis, APAGE）技术对国内99份小麦新品种（系）进行了醇溶蛋白分析。结果表明，供试材料中共分离出蛋白带2 192条，迁移率不同的谱带类型106种，其中迁移率编号为9和11的2种谱带出现频率最高，均为82.11%；有38条谱带的出现频率低于10%，这些低频率谱带仅出现在极少数的材料中；其余66条谱带出现频率为10.53%~64.21%，具有较高的多态性。每个被检测材料可电泳分离出15 ~ 30条带，其中大部分为18 ~ 24条。供试材料间遗传距离（Genetic distance, GD）为0.07 ~ 0.73，平均为0.59，遗传变异较大。聚类分析可将其分为4大类。与过去的小麦品种醇溶蛋白研究结果相比，新品种（系）的平均遗传距离缩小，遗传基础变窄。

Analysis of the Genetic Diversity at Gliadin Loci in 99 New Domestic Wheat Cultivars or Lines

In order to investigate the genetic diversity at gliadin loci in new wheat(Triticum aestivum L.) cultivars or lines,and to provide genetic information for parent selection in wheat breeding program,99 new domestic wheat cultivars or lines were analyzed by acid polyacrylamide gel electrophoresis(A-PAGE).The results showed that a total of 2 192 protein bands were identified with 106 different relative mobility rates in the examined wheat accessions.Both bands of No.9 and 11 had the highest present frequency o...