| 应用PCR技术检测伪狂犬病病毒 |
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| 引用本文： | 车勇良,俞伏松,胡奇林,程晓霞,王隆柏,魏宏,庄向生,陈少莺.应用PCR技术检测伪狂犬病病毒[J].动物医学进展,2005,26(11):81-83. |
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| 作者姓名： | 车勇良 俞伏松 胡奇林 程晓霞 王隆柏 魏宏 庄向生 陈少莺 |
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| 作者单位： | 福建省农业科学院畜牧兽医研究所,福建福州,350013;福建省畜禽疫病防治工程技术研究中心,福建福州,350013;福建省农业科学院畜牧兽医研究所,福建福州,350013 |
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| 摘 要： | 根据伪狂犬病病毒(PRV)的gE基因序列,设计并合成了一对引物,以闽A株DNA为模板,建立了检测PRV的PCR方法。该方法能从猪细小病毒闽A株和FB株中扩增出一条长度为1 808 bp的片段,对Bartha株、gE-株检测为阴性;而以猪圆环病毒、猪繁殖与呼吸综合征病毒、猪伪狂犬病病毒、猪瘟病毒和正常细胞的核酸为模板的均为阴性;敏感性试验表明,该体系可检测到10pg的猪细小病毒基因组DNA。表明该方法适用于检测猪伪狂犬病病毒野毒株或非gE基因缺失弱毒株。
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| 关 键 词： | PCR 检测 伪狂犬病病毒 |
| 文章编号： | 1007-5038（2005）11-0081-03 |
| 收稿时间： | 2005-06-13 |
| 修稿时间： | 2005年6月13日 |
The Detection of Pseudorabies virus with PCR |
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| CHE Yong-liang,YU Fu-song,HU Qi-lin,CHENG Xiao-xia,WANG Long-bai,WEI Hong,ZHUANG Xiang-sheng,CHEN Shao-ying.The Detection of Pseudorabies virus with PCR[J].Progress In Veterinary Medicine,2005,26(11):81-83. |
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| Authors: | CHE Yong-liang YU Fu-song HU Qi-lin CHENG Xiao-xia WANG Long-bai WEI Hong ZHUANG Xiang-sheng CHEN Shao-ying |
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| Institution: | 1. Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, Fuzhou, Fuj ian ,35 0013 , China ; 2. Fujian Engineering Research Center for Animal and Poultry Disease Prevention and Therapy, Fuzhou, Fujian,350013, China |
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| Abstract: | The PCR method to detect the pseudorabies virus(PRV) was established,after designed andsynthesized of a pair of pri mers based on gE sequences of the PRV genome.This PCRtechnique was ap-plied to specifically amplifythe 1808 bp DNAfragment of the PRVFa strains and FBstrains.The negativeresults were achievedfromBartha-K61,gE-strain,PK-15,porcine circovirus(PCV),porcine reproduc-tive and respiratory syndrome virus(PRRSV),Porcine parvovirus(PPV),Classical swine fever virus(CS-FV).The sensitivity of PCRreached to10 pg of PRV Fa strain DNA.The results showed that this PCRtechnique is reliable to detect wild strains and vaccine strain of PRVexcept gE-strains of PRVs. |
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| Keywords: | PCR |
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