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Sunflower mutant containing high levels of palmitic acid in high oleic background
Authors:José M. Fernández-Martínez  Manuel Mancha  Jorge Osorio  Rafael Garcés
Affiliation:(1) Instituto de Agricultura Sostenible (CSIC), Apartado 4084, 14080 Córdoba, Spain;(2) Instituto de la Grasa (CSIC), Apartado 1078, 41080 Sevilla, Spain
Abstract:A new sunflower mutant, CAS-12, was obtained, which has both high palmitic (≈30%) and high oleic acid contents, and also a substantial amount of palmitoleic acid (≈7%). The mutant was selected after X-ray irradiation of dry seeds of the inbred line BSD-2-423, which had normal palmitic (≈3%) and high oleic (≈88%) acid levels. The increase of palmitic and palmitoleic acids occurred at the expense of the oleic acid content, which decreased to around 55% in respect to the original line. Linoleic acid content is always under 5%. Palmitic and palmitoleic acid levels were similar to those of the high palmitic mutant CAS-5 obtained in a previous programme from a low oleic line isogenic to BSD-2-423 using a similar mutagenic treatment. In that previous programme we also selected three high stearic acid mutants using chemical mutagenic treatment on the same sunflower line (RDF-1-532). We attempted to obtain mutants in other lines but were unsuccessful. The isolation of similar mutants in isogenic parental lines illustrates the importance of the genetic background in the development of specific mutants with an altered seed oil fatty acid composition. The oil of this mutant will increase the range of potential uses of sunflower oil. This revised version was published online in July 2006 with corrections to the Cover Date.
Keywords:Helianthus annuus  fatty acids  palmitic acid  X-ray mutagenesis  seed oil  sunflower
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