| 垂直板小型分析型蛋白质双向电泳技术体系的改进 |
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| 引用本文： | 郭志雄,林琳,吕柳新.垂直板小型分析型蛋白质双向电泳技术体系的改进[J].福建农林大学学报(自然科学版),2006,35(2):182-186. |
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| 作者姓名： | 郭志雄 林琳 吕柳新 |
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| 作者单位： | 福建农林大学园艺学院,福建,福州,350002 |
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| 摘 要： | 以荔枝嫩叶和成熟叶为材料,第1向以小型垂直板(作胶净面积82 mm×82 mm)电泳体系进行等电聚焦,采用1.0mm厚的凝胶,通过对聚焦时间进行改进,建立了快速的小型分析型蛋白质双向电泳技术体系.应用该体系获得了稳定、清晰的双向电泳图谱.经考马斯亮蓝R-250染色显示,在荔枝嫩叶和成熟叶片中清晰可辨的蛋白质斑点数有200多个.并对该体系的应用进行了初步讨论.
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| 关 键 词： | 蛋白质 聚焦时间 双向电泳 |
| 文章编号： | 1671-5470(2006)02-0182-05 |
| 收稿时间： | 2005-05-24 |
| 修稿时间： | 2006-02-10 |
An improved mini analytical two dimensional vertical polyacrylamide electrophoresis system |
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| GUO Zhi-xiong,LIN Lin,L Liu-xin.An improved mini analytical two dimensional vertical polyacrylamide electrophoresis system[J].Journal of Fujian Agricultural and Forestry University,2006,35(2):182-186. |
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| Authors: | GUO Zhi-xiong LIN Lin L Liu-xin |
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| Institution: | GUO Zhi-xiong,LIN Lin,L(U) Liu-xin |
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| Abstract: | Young leaves and mature leaves of litchi(Litchi chinensis Sonn.) were used as materials.Focusing time of the first dimensional IEF was optimized using mini vertical slab gel(gel size: 82 mm×82 mm,gel thickness: 1.0 mm),and a rapid system of analytical two dimensional electrophoresis(2-DE) of protein was established.More than 200 protein spots visualized when stained with Coomassie Brilliant Blue R-250.Application of this mini system was discussed as well. |
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| Keywords: | protein focusing time two dimensional electrophoresis |
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