Variation in Nicotine Content of Cultured Cell Lines of Nicotiana Species and their Somatic and Sexual Hybrids |
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| Authors: | M. Sabour J. Simmonds G. Setterfield |
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| Affiliation: | Dr. M, Sabour;, Animal Research Centre, Research Branch, Agriculture Canada Dr. J. Simmonds;, Plant Research Centre, Agriculture Canada, Ottawa, Ontario, KIA OC6 Dr. G. Settterfield;, Department of Biology, Carleton University, Ottawa, Ontario, KIS 6B5 (Canada). |
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| Abstract: | Several Nicotiana species, their intergeneric sexual and somatic hybrids were compared for their in vitro growth rate and nicotine content. Cell yield on a dry weight basis and HPLC analysis of their nicotine content revealed a very high variation within each cell line from parental, sexual and somatic hybrids for the two traits analyzed. The trigeneríc sexual hybrids (N. rustica × N. debneyi) × N. tabacum and only two of the N. rustica × N. tabacum somatic hybrid lines show a positive heterosis in nicotine content in vitro The in vitro growth rate and nicotine content did not demonstrate a statistically significant correlation. |
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| Keywords: | Nicotiana spec somatic hybrids sexual hybrids genetic variability HPLC analysis |
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