棉花纤维中苯丙烷类结构单体的检测方法

苯丙烷类化合物存在于棉花纤维中,并且与纤维品质的形成有关。为正确评价棉花纤维中存在的苯丙烷类结构单体,首先采用巯基乙酸法提取出棉花纤维中的苯丙烷类化合物,然后用衍生化后的还原裂解(DFRC,Derivatization followed by reductive cleavage)法结合气相色谱(GC,Gas chromatograph)对棉花纤维中的苯丙烷类单体进行测定。结果表明,利用该方法检测到棉花纤维苯丙烷类单体主要由愈创木基结构单体(G￣木质素)和紫丁香基结构单体(S￣木质素)组成。本研究为进一步揭示棉花纤维中苯丙烷类化合物的合成奠定了基础。

A Method for Detecting Phenylpropanoid Monomers in Cotton Fiber

In the plant cell wall, the phenylpropane monomer affects the properties of the polymers derived from phenylpropanoid compounds. Recent studies have found that the phenylpropane metabolism (the second largest metabolic pathway after the glucose metabolism) exists in developing cotton fibers. Phenylpropanoid compounds present in cotton fiber are related to the formation of quality fiber. Because the phenylpropanoid compounds contents of cotton fibers are very low, and mature cotton fibers have dead, hollow, soft, dried cell walls that are difficult to triturate with conventional grinding techniques, the existing methods used to directly analyze the phenylpropanes monomer are unsuitable for use in cotton fibers. In this experiment, to evaluate phenylpropanoid monomers in cotton fibers, phenylpropanoid compounds were extracted from cotton fiber using thioglycolic acid, then using the derivatization followed by the reductive cleavage method and gas chromatograph analysis, the phenylpropanoid monomers of cotton fiber were determined. The results showed that guaiacyl monomer (G-lignin) and syringyl monomer (S-lignin) are present in cotton fibers. This study will lay a basis for further analysis of phenylpropanoid compounds in cotton fiber.