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| 亚洲璃眼蜱免疫抑制蛋白p36基因的RNA干扰研究 | |
| 引用本文： | 卢晓娟,赵素华,周勇志,张厚双,曹杰,周金林.亚洲璃眼蜱免疫抑制蛋白p36基因的RNA干扰研究[J].中国兽医寄生虫病,2011,19(3):49-53. |
| 作者姓名： | 卢晓娟 赵素华 周勇志 张厚双 曹杰 周金林 |
| 作者单位： | 1. 上海师范大学生命与环境科学学院,上海,200234;中国农业科学上海兽医研究所,上海,200241 2. 宿州职业技术学院,宿州,234101 3. 中国农业科学上海兽医研究所,上海,200241 |
| 基金项目： | 国家自然科学基金(30871846) |
| 摘 要： | 本研究利用RNA干扰技术研究亚洲璃眼蜱p36基因在其生长、发育、繁殖方面的生物学功能。通过注射p36基因的dsRNA成功实现了该基因的表达沉默,干扰组表达量下降了99%。蜱p36基因沉默后,24 h的吸附率下降了50%,饱血率下降了55.5%,卵孵化率下降了100%。结果表明p36基因在亚洲璃眼蜱吸血和繁殖中具有重要作用。 |
| 关 键 词： | 亚洲璃眼蜱 免疫抑制蛋白 RNA干扰 |
STUDY ON RNA INTERFERENCE OF THE IMMUNOSUPPRESSANT PROTEIN P36 IN THE TICK HYALOMMA ASIATICUM |
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| LU Xiao-juan , ZHAO Su-hua , ZHOU Yong-zhi , ZHANG Hou-shuang , CAO Jie , ZHOU Jin-lin.STUDY ON RNA INTERFERENCE OF THE IMMUNOSUPPRESSANT PROTEIN P36 IN THE TICK HYALOMMA ASIATICUM[J].Chinese Journal of Veterinary Parasitology,2011,19(3):49-53. | |
| Authors: | LU Xiao-juan ZHAO Su-hua ZHOU Yong-zhi ZHANG Hou-shuang CAO Jie ZHOU Jin-lin |
| Institution: | LU Xiao-juan1,2,ZHAO Su-hua3,ZHOU Yong-zhi2,ZHANG Hou-shuang2,CAO Jie2,ZHOU Jin-lin2(1.College of Life and Environment Science,Shanghai Normal University,Shanghai 200234,China,2.Shanghai Veterinary Research Institute,CAAS,Shanghai 200241,3.College of Vocational and Technical of Suzhou,Suzhou 234101,China) |
| Abstract: | In this study,the functions of p36 gene of Hyaloma asiaticum(Ha-p36) in growth,development and reproduction were studied using RNA interference(RNAi).By the injection of double-strand RNA(dsRNA) of Ha-p36,gene silencing was confirmed by real-time PCR,which showed the expression amount of Ha-p36 gene decreased 99% after RNAi.Comparing with the control,the tick attachment rate,engorgement rate and egg hatching rate decreased 50%,55.5% and 100% in the RNAi group,respectively.The results indicated that Ha-p36 m... |
| Keywords: | Hyalomma asiaticum immunosuppressive protein RNA interference(RNAi) |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |