| 两株禽腺病毒人工感染产蛋鸡后脏器组织中病毒分布及其含毒量的检测 |
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| 引用本文： | 朱国强,王永坤,郭建荣,金建,王东华,张彪,郑玉美.两株禽腺病毒人工感染产蛋鸡后脏器组织中病毒分布及其含毒量的检测[J].扬州大学学报(农业与生命科学版),1993(3). |
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| 作者姓名： | 朱国强 王永坤 郭建荣 金建 王东华 张彪 郑玉美 |
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| 作者单位： | 江苏农学院牧医系，江苏农学院牧医系，江苏农学院牧医系，江苏农学院牧医系，江苏农学院牧医系，江苏农学院牧医系，江苏农学院牧医系 扬州 225001，扬州 225001，扬州 225001，扬州 225001，扬州 225001，扬州 225001，扬州 225001 |
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| 摘 要： | 分别采用H_(91-1)和Y_(81)G_1两株禽腺病毒通过滴鼻、口服和肌肉注射,人工感染SPF产蛋鸡、伊莎蛋鸡,在感染后第3～20天的不同时期,分别扑杀感染鸡,无菌采集各脏器组织,应用荚心ELISA和血凝(HA)试验检测病料及鸭胚分离病毒。结果表明,H_(91-1)和Y_(81)G_4两株禽腺病毒对SPF蛋鸡和普通蛋鸡的咽喉部、卵巢、输卵管(蛋白分泌部、峡部、子宫部)均有亲嗜性。最早在感染后第3天即能检出病毒,感染后第6～15天检出率最高,输卵管峡部是检测和分离病毒的最佳部位。
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| 关 键 词： | 鸡 禽腺病毒 检测 |
DETECTION AND DISTRIBUTION OF EDS VIRAL ANTIGEN IN LAYING HENS TISSUES POST EXPERIMENTAL INFECTION WITH NEW STRAINS H_(91-1) AND Y_(81)G_4 OF EDS_(76) VIRUS |
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| ZHU Guoqiang WANG Yongkun GUO Jianyong JIN Jian WANG Tonghua ZHANG Biao ZHENG Yumei.DETECTION AND DISTRIBUTION OF EDS VIRAL ANTIGEN IN LAYING HENS TISSUES POST EXPERIMENTAL INFECTION WITH NEW STRAINS H_(91-1) AND Y_(81)G_4 OF EDS_(76) VIRUS[J].Journal of Yangzhou University:Agricultural and Life Science Edition,1993(3). |
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| Authors: | ZHU Guoqiang WANG Yongkun GUO Jianyong JIN Jian WANG Tonghua ZHANG Biao ZHENG Yumei |
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| Abstract: | Conventionally reared and SPF laying hens were infected with new two strains H_(91-1) and Y_(81)G_4 of EDS_(76) virus associated with an eggdrop syndrome in chickens and killed at intervals during the first 20 days post inoculation (Pi). Tissues were collected and studied using haemagglutination inhibition test, sand-wich ELISA test and embryonating duck eggs inoculation for presence of haemagglutinating EDS_(76). It was shown that both H_(91-1) and Y_(81)G_4 EDS_(76) Viral antigen were detected in the pharynx, ovary, oviduct 20 days pi and reached a~+ high level 6 to 15 days pi. Viral antigen was first observed in the pharynx and isthmus of ovidus as early as 3 days pi. The findings suggest virus isolation best attempt using the isthmus. |
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| Keywords: | Chicken Avian adenovirus Detection |
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