豌豆属种质资源遗传多样性的ISSR分析

用ISSR标记技术对来自国内外的73份豌豆材料进行遗传多样性分析,结果表明,100个ISSR引物中共筛选出11个多态性明显、条带清晰、反应稳定的引物,73份材料DNA共扩增出91条条带,其中78条为多态性条带,平均每个引物扩增的条带数为8.2条,多态性比率为86.4%。Shannon多样性指数平均为0.420 2,每个位点的有效等位基因数为1.451 8,品种间遗传相似系数变幅为0.406 5～0.934 0,表现出丰富的遗传多样性。利用UPGMA聚类分析,以遗传相似系数0.52为界限,73份材料划分为5类,聚类基本符合地理来源相近的材料聚为一类,呈现出一定的地域性分布规律。

Genetic diversity of Pisum sativum germplasm resources by ISSR

The genetic diversity of the 73 materials of Pisum sativum from both at home and abroad were analyzed by ISSR marker.11 primers were selected from 100 primers which have the clearly and polymorphic bands,a total of 91 bands were obtained by 73 materials,including 78 polymorphism bands.On average,each primer amplification site for 8.2 and the polymorphism percentage was 86.4%.Shannon’s index was 0.420 2 in average and the number of effective alleles was 1.451 8,the genetic similarity coefficient ranged from 0.406 5 to 0.934 0 and shows rich genetic diversity.Use groups by UPGMA cluster analysis to the genetic similarity coefficient 0.52 for boundaries,73 materials are divided into five types,clustering basic accord with geographical origin of close materials for a class together,has a certain regional distribution.