Flow cytometric detection of bovine viral diarrhea virus in peripheral blood leukocytes of persistently infected cattle. |
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| Authors: | P Qvist, B Aasted, B Bloch, A Meyling, L R nsholt, H Houe |
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| Affiliation: | Animal Biotechnology Research Center, National Veterinary Laboratory, Copenhagen, Denmark. |
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| Abstract: | Flow cytometry was investigated for detection of bovine viral diarrhea virus (BVDV) in peripheral blood mononuclear leukocytes of persistently infected cattle. The mononuclear leukocytes were purified by sedimentation in a gradient of Ficoll-Paque, fixed, permeabilized, and then labelled by indirect immunofluorescence using biotinylated immunoglobulins from a porcine antiserum to BVDV. Flow cytometric analysis of blood samples obtained from persistently infected cattle revealed virus in 3.0-21.0% (mean +/- SD, 11.2% +/- 6.4%) of the mononuclear leukocytes. Fluorescent cells were not observed in controls. Flow cytometric detection of BVDV in blood cells of persistently infected bovines is a rapid and objective technique which does not require cell culture facilities. |
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