Binding of mycoplasmas to solid phase adsorbents |
| |
Authors: | Szathmáry Susan Rajapakse Nandani Székely Ibolya Pitlik E Bíró Judit Erdei Noémi Stipkovits L |
| |
Institution: | Clarigen Inc, Carlsbad, CA, USA. |
| |
Abstract: | The capture of mycoplasmas (M. hominis, M. buccale, M. fermentans, M. bovis, M. synoviae, M. gallisepticum and M. arthritidis) based on lipid structures and adhesion molecules present in the mycoplasmal membrane was tested using different chromatographic resins (ActiClean Etox, ClarEtox, Heparin-Actigel, Sulfated Hiflow and SulfEtox). All of the resins efficiently reduced mycoplasma concentrations in Phosphate Buffered Saline (PBS) and in Fetal Bovine Serum (FBS) by 3-8 logs in a few minutes. This technology could be used for removing mycoplasmas from tissue culture components such as serum, and for concentrating mycoplasmas in vaccine production. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|