中药地翁颗粒对小鼠脾淋巴细胞功能的影响 |
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引用本文: | 夏方,冷春青,付运星,吴永丽,郭勋,董海兵,张立艳,白换力,吴帅成,韦旭斌,伊鹏霏,付本懂.中药地翁颗粒对小鼠脾淋巴细胞功能的影响[J].兽医大学学报,2014(8):1341-1344. |
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作者姓名: | 夏方 冷春青 付运星 吴永丽 郭勋 董海兵 张立艳 白换力 吴帅成 韦旭斌 伊鹏霏 付本懂 |
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作者单位: | 吉林大学动物医学学院,吉林长春130062 |
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基金项目: | 国家自然科学基金资助项目(31372470) |
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摘 要: | 为观察中药地翁颗粒在体外对小鼠脾淋巴细胞功能的影响,设置高(100mg/L)、中(10mg/L)、低(1mg/L)3个剂量组,采用3 H法进行刀豆蛋白A(ConA)、脂多糖(LPS)诱导脾淋巴细胞增殖试验,ELISA方法检测ConA或金黄色葡萄球菌Cown株灭活菌体(SAC)刺激的脾淋巴细胞上清液IL-2、IL-6、IL-10、IL-12、IFN-γ和TNF-α的含量。结果显示,在安全浓度范围内,地翁颗粒能够明显促进T淋巴细胞和B淋巴细胞增殖;能够促进ConA刺激的淋巴细胞分泌INF-γ;能够促进SAC刺激的淋巴细胞分泌IL-12、INF-γ和IL-6,并抑制IL-10的分泌。结果表明,地翁颗粒能够有效调节小鼠脾淋巴细胞的功能,促进细胞免疫。
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关 键 词: | 小鼠 地翁颗粒 中药 脾淋巴细胞 |
Effect of Diweng Keli on immune function of mouse spleen lymphocyte |
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Authors: | XIA Fang LENG Chun-qing FU Yun-xing WU Yong-li GUO Xun DONG Hai-bin ZHANG Li-yan BAI Huan-li WU Shui-cheng WEI Xu-bin YI Peng-fei FU Ben-dong |
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Institution: | (College of Veterinary Medicine , J ilin University ,Changchun 130062 ,China) |
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Abstract: | To examine the effect of Diweng Keli on immune function of mouse spleen lymphocyte in vitro,the blastogenesis of the responder cells was quantified by a colorimetric method using MTT in 96-well microculture plates. Then,we investigated the effects of Diweng Keli on the proliferative activity of lymphocytes stimulated by concanavalin A(Con A) and lipopolysaccharide(LPS) by 3 H- thymidine and cytokine levels in the supernatant by ELISA. The results showed that Diweng Keli promotes the proliferative activity of lymphocytes stimulated by concanavalin A(Con A)and li- popolysaccharide(LPS) ,the production of IFN-)' of T cells and the production of IL-12,IFN-7 and IL-6 of antigen-presenting cells(APC) ,but inhibits the production of IL-10 of APC. These results indicated that Diweng Keli promotes cell-mediated immunity of mice and regulate mice immune function. |
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Keywords: | mouse Diweng Keli traditional Chinese medicine spleen lymphocyte |
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