| 副猪嗜血杆菌tbpB基因的PCR-RFLP分型 |
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| 引用本文： | 李淑芳,龚玉梅,侯娜,赵成全,赵兴绪,张培君.副猪嗜血杆菌tbpB基因的PCR-RFLP分型[J].中国畜牧兽医,2013,0(7):56-61. |
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| 作者姓名： | 李淑芳 龚玉梅 侯娜 赵成全 赵兴绪 张培君 |
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| 作者单位： | 1. 甘肃农业大学动物医学院, 甘肃兰州 730046;2. 北京市农林科学院畜牧兽医研究所, 北京 100097 |
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| 基金项目： | “863”国家高技术研究发展计划项目(2011AA10A210);国家自然科学基金青年基金(31001072) |
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| 摘 要： | 本研究应用生物学软件Vector NTI对副猪嗜血杆菌(Haemophilus parasuis,Hps)的转铁结合蛋白tbpB进行基因分型研究,通过选择AluⅠ、AvaⅡ和RsaⅠ3种限制性内切酶对15株Hps标准株和12株分离株的tbpB基因进行RFLP分析。经过生物学软件Vector NTI精确分型,标准株得到了11个基因型,其中基因型Ⅰ(AAA)包括血清型1、3和15,Ⅱ(BBB)包括血清型2、9和11;分离株的分型产生了10种基因型,其中6种为新的基因型。这一结果证实了Hps流行株的多样性,说明该方法对于猪革拉泽氏病流行病学规律的研究具有实用意义,同时,需要对更多的分离株做进一步的研究。
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| 关 键 词： | 副猪嗜血杆菌 tbpB基因 PCR-RFLP 生物学软件Vector NTI |
| 收稿时间： | 2012-11-27 |
Typing of Haemophilus parasuis Strains tbpB Gene by PCR-RFLP Analysis |
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| LI Shu-fang,GONG Yu-mei,HOU Na,ZHAO Cheng-quan,ZHAO Xing-xu,ZHANG Pei-jun.Typing of Haemophilus parasuis Strains tbpB Gene by PCR-RFLP Analysis[J].China Animal Husbandry & Veterinary Medicine,2013,0(7):56-61. |
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| Authors: | LI Shu-fang GONG Yu-mei HOU Na ZHAO Cheng-quan ZHAO Xing-xu ZHANG Pei-jun |
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| Institution: | 1. College of Animal Medicine, Gansu Agricultural University, Lanzhou 730046, China;2. Institute of Animal Husbandry and Veterinary Medicine of Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China |
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| Abstract: | Using molecular biology software Vector NTI, the transferring-binding proteins-B of 15 Haemophilus parasuis(Hps) reference serovars and 12 isolates were analyzed with AluⅠ,AvaⅡ and RsaⅠ endonucleases. Reference serovars produced 11 different genotypes. GenotypeⅠ(AAA) included serovar 1,3,15 and genotypeⅡ(BBB) was represented by serovar 2, 9,11. Isolates had 10 genotypes, and 6 of those were new. The fact suggested heterogeneity of Hps isolates and PCR-RFLP was an useful method for studing the epidemiology of outbreaks of Glasser’s disease. Therefore, further validation with more field strains was needed. |
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| Keywords: | Haemophilus parasuis tbpB gene PCR-RFLP molecular biology software Vector NTI |
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