| 不同温度下2种沼虾磷酸酶活性变化的研究 |
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| 引用本文： | 苏翔,汪桂玲,李家乐.不同温度下2种沼虾磷酸酶活性变化的研究[J].水产科学,2011,30(3):168-170. |
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| 作者姓名： | 苏翔 汪桂玲 李家乐 |
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| 作者单位： | 1. 上海海洋大学,省部共建水产种质资源发掘与利用教育部重点实验室,上海,201306
2. 上海海洋大学,省部共建水产种质资源发掘与利用教育部重点实验室,上海,201306;上海市高校水产养殖学E研究院,上海,201306 |
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| 基金项目： | 上海市科委地方院校能力建设项目，上海市水产养殖重点学科建设项目资助 |
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| 摘 要： | 用比色法测量4℃和-70℃下离体的罗氏沼虾和日本沼虾肝胰腺中的碱性磷酸酶、酸性磷酸酶酶活性在一周内的变化。试验结果表明,4℃条件下保存的日本沼虾与罗氏沼虾的离体组织中碱性磷酸酶和酸性磷酸酶酶活性有效测定下时间为3 d,-70℃下有效测定时间为2 d,对后续酶活性试验没有大的影响。
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| 关 键 词： | 日本沼虾 罗氏沼虾 碱性磷酸酶 酸性磷酸酶 有效测定时间 |
Variation in Activities of Phosphatase in Prawn Macrobrachium nipponense and M. rosenbergii under Different Water Temperature |
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| SU Xiang,WANG Gui-ling,LI Jia-le.Variation in Activities of Phosphatase in Prawn Macrobrachium nipponense and M. rosenbergii under Different Water Temperature[J].Fisheries Science,2011,30(3):168-170. |
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| Authors: | SU Xiang WANG Gui-ling LI Jia-le |
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| Institution: | SU Xiang1,WANG Gui-ling1,LI Jia-le 1,2(1.Key Laboratory of Exploitation and Utilization of Aquatic Genetic Resources,Shanghai Ocean University,Ministry of Education,Shanghai 201306,China,2.Aquaculture Division,E-Institute of Shanghai Universities,China) |
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| Abstract: | Acid phosphatase(ACP)and alkaline phosphatase(AKP)as two important regulatory enzymes involved in metabolism and protection in a body are generally stored and transported at 4 ℃ and-70 ℃ in in vitro or tissues of aquatic organisms.Colorimetric method was used to determined activities of the two enzymes in hepatopancreas in prawn Macrobrachium nipponense and M.rosenbergii at 4 ℃ and-70 ℃ for a week.It was found that the activities of the two enzymes were effectively detected in 3 days at 4 ℃ and in 2 days at-70 ℃ without significant effect thereafter. |
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| Keywords: | Macrobrachium nipponense Macrobrachium rosenbergii ACP AKP effective detecting time |
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