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响应面法优化姬松茸液体发酵培养基中红糖和酵母膏浓度
引用本文:范雷法,潘慧娟,Soccol CR,Pandey A.响应面法优化姬松茸液体发酵培养基中红糖和酵母膏浓度[J].食用菌学报,2005,12(3):27-31.
作者姓名:范雷法  潘慧娟  Soccol CR  Pandey A
作者单位:1. 浙江省农业科学院园艺研究所,杭州,310021
2. Laboratory of Biotechnological Process, Federal University of Parana,Curitiba-PR,81531-970, Brazil
3. Biotechnology Division, Regional Research Laboratory,Trivandrurn-695 019,India
基金项目:浙江农科院启动项目“姬松茸胞外多糖优化生产和深度开发研究”(编号:2003-B0201)的部分研究内容
摘    要:运用响应面分析法优化姬松茸液体发酵培养基中红糖和酵母膏浓度.结果表明,红糖浓度为20.24g/L、酵母膏浓度为2.055g/L时,菌丝体生物量最高,达2.279g/L;红糖浓度为20.8g/L、酵母膏浓度为2.23g/L时,胞外多糖产量最高,达0.749g/L.

关 键 词:响应面分析法  姬松茸  红糖  酵母膏
文章编号:1005-9873(2005)03-0027-05
收稿时间:2005-04-13
修稿时间:2005-07-13

Response Surface Methodology for Optimization of the Concentration of Brown Sugar and Yeast Extract for Mycelial Biomass and Exopolysaccharide Yield of Agaricus blazei
FAN Lei-fa,PAN Hui-juan,Soccol CR,Pandey A.Response Surface Methodology for Optimization of the Concentration of Brown Sugar and Yeast Extract for Mycelial Biomass and Exopolysaccharide Yield of Agaricus blazei[J].Acta Edulis Fungi,2005,12(3):27-31.
Authors:FAN Lei-fa  PAN Hui-juan  Soccol CR  Pandey A
Institution:1 Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 2 Laboratory of Biotechnological Process, Federal University of Parana, Curitiba-PR, 81531-970, Brazil; 3 Biotechnology Division, Regional Research Laboratory, Trivandrum-695 019, India
Abstract:Response surface experiments were conducted on Agar/cus blazei to determine the optimal concentration of brown sugar and yeast extract for mycelial biomass and exopolysaccharide yield. The results showed that the mycelial biomass could reach of 2. 279g/L when the concentrations of brown sugar and yeast extract were 20.24g/L and 2. 055g/L respectively. And the exopolysaccharide yield could reach of 0. 749g/L when the concentrations of brown sugar and yeast extract were 20.8g/L and 2.23g/L respectively.
Keywords:Response surface analysis  Agaricus blazei  Brown sugar  Yeast extract
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