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| 玉米基因sbe1 cDNA的克隆与原核表达 | |
| 引用本文： | 张军杰,周会,胡育峰,田孟良,刘汉梅,黄玉碧.玉米基因sbe1 cDNA的克隆与原核表达[J].核农学报,2009,23(1):70-74. |
| 作者姓名： | 张军杰 周会 胡育峰 田孟良 刘汉梅 黄玉碧 |
| 作者单位： | 1. 四川农业大学玉米研究所,四种,雅安,625014;四川农业大学生命理学院,四川,雅安,625014 2. 四川农业大学玉米研究所,四种,雅安,625014 3. 四川农业大学农学院,四川,雅安,625014;四川农业大学农学院,四川,雅安,625014 |
| 基金项目： | 国家高技术研究发展计划(863计划)，教育部长江学者和创新团队发展计划，四川省教育厅青年基金 |
| 摘 要： | 采用RT-PCR技术克隆了玉米基因sbe1 全长cDNA序列，在NCBI上序列比对后与文献报道的sbe1基因序列同源性达到99%。同时构建了原核生物表达载体pET32(a+)-sbe1，成功表达出了SBEⅠ蛋白。为进一步体外研究SBEⅠ的物理化学性质及催化机理奠定基础。 |
| 关 键 词： | 玉米 sbe1基因 基因表达 |
| 收稿时间： | 2008-04-23 |
Clone and Expression of sbe1 cDNA from Maize in E.coil |
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| ZHANG Jun-jie,ZHOU Hui,HU Yu-feng,TIAN Meng-liang,LIU Han-mei,HUANG Yu-bi.Clone and Expression of sbe1 cDNA from Maize in E.coil[J].Acta Agriculturae Nucleatae Sinica,2009,23(1):70-74. | |
| Authors: | ZHANG Jun-jie ZHOU Hui HU Yu-feng TIAN Meng-liang LIU Han-mei HUANG Yu-bi |
| Institution: | 1;2;1;3;1;2;1;3;1.Maize Research Institute;Sichuan Agricultural University;Ya'an;Sichuan 625014;2.Life Science College;3.Agronomy College;Sichuan 625014 |
| Abstract: | The cDNA of sbe1 from maize was cloned by RT-PCR and compared with the reported sequence of sbe1 cDNA in GenBank.The results showed that the nuleotide sequence homology were 99%.The sbe1 cDNA were inserted pET32a(+) vector by restriction endonuclease digestion, electrophoresis, gel reclamation DNA Ligation and so on molecular biological technologies, and that which were transformed to E.coil BL21(DE3), At the same time sbe1 cDNA was induced expression in E.coil BL21(DE3) by IPTG, and the SBEⅠprotein was expressed. The experimental result lay a foundation for further study on the physical and chemical properties of SBEⅠ in vitro. |
| Keywords: | maize sbe1 gene expression |
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