| ISSR标记技术及其在作物遗传育种中的应用 |
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| 引用本文： | 孙洪,程静,詹克慧,张相武,杨艳会. ISSR标记技术及其在作物遗传育种中的应用[J]. 分子植物育种, 2005, 3(1): 123-127 |
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| 作者姓名： | 孙洪 程静 詹克慧 张相武 杨艳会 |
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| 作者单位： | 河南农业大学农学院,郑州,450002;河南西华县农科所,西华县,466600 |
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| 摘 要： | ISSR(inter-simple sequence repeat)标记技术是在SSR基础上发展起来的一项新的标记技术，由于其引物设计比SSR简单，不需要知道SSR两端的碱基序列，多态性高，重复性好，能够提供更多的信息，已在多种动植物的种质鉴定、遗传作图、基因定位、遗传多样性等研究方面得到应用。本文对ISSR技术的特点、实验操作及其在作物遗传育种领域的应用进行了概述。
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| 关 键 词： | ISSR 分子标记 作物遗传育种 应用 |
ISSR Marker and its Application in Crop Genetics and Breeding |
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| Sun Hong Cheng Jing Zhan Kehui Zhang Xiangwu Yang Yanhui College of Agronomy,Henan Agricultural University,Zhengzhou,, Xihua Research Institute of Agricultural Sciences in Henan Province,Xihua County, Corresponding author,kh@.com. ISSR Marker and its Application in Crop Genetics and Breeding[J]. Molecular Plant Breeding, 2005, 3(1): 123-127 |
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| Authors: | Sun Hong Cheng Jing Zhan Kehui Zhang Xiangwu Yang Yanhui College of Agronomy Henan Agricultural University Zhengzhou Xihua Research Institute of Agricultural Sciences in Henan Province Xihua County Corresponding author kh@.com |
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| Affiliation: | Sun Hong1 Cheng Jing1 Zhan Kehui1* Zhang Xiangwu2 Yang Yanhui11 College of Agronomy,Henan Agricultural University,Zhengzhou,450002, 2 Xihua Research Institute of Agricultural Sciences in Henan Province,Xihua County,466600* Corresponding author,kh486@163.com |
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| Abstract: | Recently, inter-simple sequence repeat (ISSR) markers have emerged as an alternative system with reliability and advantages of microsatellites(SSR). ISSR primer can be designed without the knowledge about microsatellite regions, and its design is easer than that of SSR. ISSR marker has good polymorphism and repetition, and can provide more information than others. Its technique has been widely used in the studies of germplasm identification, genetic mapping, gene tagging, genetic diversity in animal and plant. This paper emphasizes on the characteristics and procedures of ISSR and its application in crop genetics and breeding. |
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| Keywords: | ISSR |
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