| 小菜蛾细胞色素P450基因的cDNA片段克隆及其序列分析 |
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| 引用本文： | 李洪山,戴华国,王娟. 小菜蛾细胞色素P450基因的cDNA片段克隆及其序列分析[J]. 江苏农业学报, 2006, 22(1): 29-32 |
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| 作者姓名： | 李洪山 戴华国 王娟 |
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| 作者单位： | 南京农业大学植物保护学院,江苏,南京,210095;江苏省盐城市农业科学院,江苏,盐城,224002;南京农业大学植物保护学院,江苏,南京,210095 |
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| 摘 要： | 以小菜蛾[Plutella xylostella(L.)]3龄幼虫(对氰戊菊酯的LC50为38.75 mg/L)的总RNA为模板,利用简并引物,采用反转录-多聚酶链式反应(RT-PCR)方法扩增出1个长度为240 bp的cDNA片段。序列分析结果表明,扩增出的cDNA片段与细胞色素P450的Cyp6基因有较高的同源性。
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| 关 键 词： | 小菜蛾 细胞色素P450基因 cDNA片段克隆 序列分析 |
| 文章编号： | 1000-4440（2006）01-0029-04 |
| 收稿时间： | 2005-03-09 |
| 修稿时间： | 2005-03-09 |
Cloning and Sequence Analysis of a cDNA Fragment of Cytochrome P450 from the Diamondback Moth, Plutella xylostella (L.) |
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| LI Hong-shan,DAI Hua-guo,WANG Juan. Cloning and Sequence Analysis of a cDNA Fragment of Cytochrome P450 from the Diamondback Moth, Plutella xylostella (L.)[J]. Jiangsu Journal of Agricultural Sciences, 2006, 22(1): 29-32 |
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| Authors: | LI Hong-shan DAI Hua-guo WANG Juan |
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| Affiliation: | 1. Department of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China ; 2. Yancheng Academy of Agricultural Sciences, Yancheng 224002, China |
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| Abstract: | A new cDNA fragment about 240 bp of cytochrome P450 was amplified by RT-PCR from total RNA of 3-intar diamondback moth,(Plutella Xylostella),a susceptible strain with LC_(50) of 38.75 mg/L to fenvalerate in laboratory condition.Sequence analysis showed that there is a high similarity between the fragment of cDNA and Cyp6 family. |
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| Keywords: | diamondback moth(Plutella xylostella) cytochrome P450 cloning of cDNA fragment sequence analysis |
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