液相色谱-串联质谱法测定鸡肝脏和肌肉中地克珠利残留量

用液相色谱-串联质谱的方法建立鸡组织中地克珠利残留的检测方法，并考察地克珠利在鸡组织 中的消除规律。用乙腈和乙酸乙酯的混合溶液提取，60℃氮气吹干，乙腈溶解，过0.22 μm微孔滤膜后， 采用液相色谱-串联质谱分析。地克珠利在鸡肝中8~4000 μg/kg 范围内具有较好的线性关系（r2>0.99）， 而在鸡肌肉中8~1200 μg/kg 范围内具有较好的线性关系（r2>0.99），检测限和定量限分别为0.5 μg/kg 和 0.8 μg/kg。鸡肝脏和肌肉中地克珠利的液相色谱串联质谱测定方法简单、灵敏度高，适用于鸡组织中地 克珠利残留量的检测。

Determination of residues of the diclazuril in chicken liver and muscle by high performance liquid chromatograpy tandem mass spectrometry

Objective：we present a method based on liquid chromatography tandem mass spectrometry for determining residues of diclazuril in chicken tissues and investigate the depletion of diclazuril in chicken tissues.Method：The analyte was extracted by mixed solution of acetonitrile and ethyl-acetate;the extracts were dried by nitrogen gas,redissolved in acetonitrile,filtered through 0.22 μ m micropore film and directly injected into the LC-MS/MS system.Result：The calibration curves were linear （r 2 0.99） in the range of 8～ 4000 μg/kg in chicken liver and 8～1200 μg/kg in chicken muscle.Limit of detection （LOD） and limit of quantitation （LOQ） were 0.5 μg/kg and 0.8 μg/kg.Conclusion：The method was simple and accurate enough to determine residues of diclazuril in chicken tissues.