马蔺叶中黄酮的超声提取工艺优化及抗氧化性能的测定 |
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引用本文: | 姜显光,侯冬岩,翁霞,王翠艳. 马蔺叶中黄酮的超声提取工艺优化及抗氧化性能的测定[J]. 现代农业科技, 2014, 0(2) |
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作者姓名: | 姜显光 侯冬岩 翁霞 王翠艳 |
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作者单位: | 鞍山师范学院化学与生命科学学院; |
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摘 要: | 在30℃条件下,采用L9(34)正交试验优化马蔺叶中黄酮的最佳提取工艺,最佳提取条件如下:乙醇浓度60%,料液比1∶30,提取次数3次,提取时间30 min。在此条件下,测得马蔺叶中黄酮的含量为9.25 mg/g,回收率为93.15%~103.92%,变异系数小于2.32%。用流动注射化学发光法试验发现,马蔺叶的抗氧化性能较强。
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关 键 词: | 马蔺 黄酮 超声波法 抗氧化性 流动注射化学发光 |
Process Optimization for Ultrasonic Extraction of Lavonoids and Determination of Antioxidation Effect in Iris lactea Pall. Var. chinensis Koidz |
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Abstract: | Under the condition of 30 ℃,the orthogonal test of L9(34)was used to optimize the extracting process for flavonoids in Iris lactea Pall. Var. chinensis Koidz,the most optimizational condition was as follows :ethanol concentration was 60%,the ratio of raw material and solvent was 1 ∶30, extraction times was 3,extraction time was 30 minutes.Under this condition,concent of flavonoids in Iris lactea Pall. Var. chinensis Koidz was 9.25 mg/g, the recoveries were 93.15%~103.92%,the coefficient of variation was no more than 2.32%. The antioxidation effect of Iris lactea Pall. Var. chinensis Koidz was determinated by the flow-injection chemiluminescence. The results showed that Iris lactea Pall. Var. chinensis Koidz had a strong antioxidation effect. |
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Keywords: | Semen Iris Pall.Var.chinensis Koidz flavonoids ultrasonic method antioxidation effect flow-injection chemiluminescence |
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