| 齿瓣石斛组织培养技术研究 |
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| 引用本文： | 高燕,李守岭,白燕冰,李泽生,耿秀英. 齿瓣石斛组织培养技术研究[J]. 云南热作科技, 2010, 0(2): 23-25,31 |
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| 作者姓名： | 高燕 李守岭 白燕冰 李泽生 耿秀英 |
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| 作者单位： | 云南省德宏热带农业科学研究所,云南瑞丽678600 |
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| 摘 要： | 以齿瓣石斛蒴果为外植体进行胚培养的试验表明,初始萌发培养基为1/2MS;原球茎萌发培养基为3/4MS＋10%马铃薯汁＋5%香蕉汁＋6-BA0.2mg·L^-1＋NAA0.05mg·L^-1＋0.05%AC;继代增殖培养基为MS＋10%马铃薯汁＋10%香蕉汁＋6-BA0.5mg.L^-1＋NAA0.2mg.L^-1＋0.1%AC;壮苗生根培养基为1/2MS＋10%香蕉汁＋5%马铃薯汁＋6-BA0.2mg·L^-1＋NAA1.0mg·L^-1＋0.1%AC,生根诱导率达100%,成活率达90%以上。
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| 关 键 词： | 齿瓣石斛 种胚 组织培养 |
Study on Dendrobium devonianum in Vitro |
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| GAO Yan,LI Shou-ling,BAI Yan-bing,LI Ze-sheng,GENG Xiu-ying. Study on Dendrobium devonianum in Vitro[J]. Journal of Yunnan Tropical Crops Science & Technology, 2010, 0(2): 23-25,31 |
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| Authors: | GAO Yan LI Shou-ling BAI Yan-bing LI Ze-sheng GENG Xiu-ying |
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| Affiliation: | (Dehong Institute of Tropical Agriculture, Ruili 678600, China ) |
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| Abstract: | Experiment of Dendrobium devonianum was carded out in Dehong. Results indicated that the optimum medium were 3/4MS＋6- BA0.2 mg.L^-1 NAA0.05 mg.L^-1＋10% banana juice＋5 % potato juice, MS＋6-BA0.5 mg.L^-＋NAA0.2 mg.L^-＋10% banana juice＋ 10% potato juice and I/2MS ＋6-BA0.2 mg.L^- ＋NAA1.0 mg.L^-＋10% banana juice ＋5% potato juice for seedling, propagation culture and rooting respectively. Root inducing rate reaches 100% and survival rate is up to 90% after transplanting. |
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| Keywords: | Dendrobium devonianum embryo breeding tissue culture |
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