Production and analysis of reciprocal hybrids between <Emphasis Type="Italic">Asparagus officinalis</Emphasis> L. and <Emphasis Type="Italic">A. schoberioides</Emphasis> Kunth |
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Authors: | Takuro Ito Toshinori Ochiai Hiroki Ashizawa Toshinori Shimodate Takahiro Sonoda Tatsuya Fukuda Jun Yokoyama Toshiaki Kameya Akira Kanno |
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Institution: | (1) Graduate School of Life Sciences, Tohoku University, Katahira 2-1-1, Aoba-ku, Sendai 980-8577, Japan;(2) Graduate School of Science, Hiroshima University, Kagamiyama 1-4-3, Higashi-Hiroshima 739-8526, Japan;(3) Fukushima Prefecture Aizubange Agricultural Dedication, Mimyou Minamihara 881, Aizubange 969-6506, Japan;(4) Graduate School of Life Sciences, Tohoku University, Aoba, Aoba-ku, Sendai 980-8578, Japan |
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Abstract: | The genus Asparagus (Asparagaceae) encompasses 100–300 species, including the important vegetable crop, A. officinalis (garden asparagus). Previous attempts to hybridize garden asparagus with A. densiflorus (Kunth) Jessop, with the aim of introducing disease resistance, were unsuccessful because of the failure of endosperm development.
In the present study, reciprocal interspecific hybrids between garden asparagus and A. schoberioides Kunth were generated by hand pollination. The F1 hybrids were analyzed by using both morphological and molecular techniques.
This is the first report describing the production of an interspecific hybrid between garden asparagus at the diploid level
(2n = 2x = 20) and its diploid (2n = 2x = 20) wild relative, A. schoberioides. Morphological characteristics of candidate hybrids were a mixture of those found in the parents, and cytological and RFLP
analyses confirmed that morphologically intermediate plants were indeed diploid hybrids of those two species. In other words,
post-zygotic isolation is not complete between phylogenetically distinct these two species. Our results suggest that other
Asparagus species may be capable of hybridizing with A. officinalis and that introducing wild characters through interspecific hybridization may offer advantages for breeding for novel traits.
Takuro Ito and Toshinori Ochiai authors are contributed equally to this work |
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Keywords: | Asparagus officinalis Asparagus schoberioides Chromosome number Flow cytometry Interspecific hybridization Morphological analysis Reciprocal crosses Reproductive barrier Restriction fragment length polymorphism (RFLP) |
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