| 甘蔗与黑穗病菌互作的叶片差异蛋白分析 |
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| 引用本文： | 阙友雄,林剑伟,徐景升,阮秒鸿,许莉萍,张木清,陈由强,陈如凯. 甘蔗与黑穗病菌互作的叶片差异蛋白分析[J]. 热带作物学报, 2008, 29(2): 136-140 |
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| 作者姓名： | 阙友雄 林剑伟 徐景升 阮秒鸿 许莉萍 张木清 陈由强 陈如凯 |
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| 作者单位： | 农业部甘蔗生理生态与遗传改良重点开放实验室,福州,50002;农业部甘蔗生理生态与遗传改良重点开放实验室,福州,50002;农业部甘蔗生理生态与遗传改良重点开放实验室,福州,50002;农业部甘蔗生理生态与遗传改良重点开放实验室,福州,50002;农业部甘蔗生理生态与遗传改良重点开放实验室,福州,50002;农业部甘蔗生理生态与遗传改良重点开放实验室,福州,50002;农业部甘蔗生理生态与遗传改良重点开放实验室,福州,50002;农业部甘蔗生理生态与遗传改良重点开放实验室,福州,50002 |
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| 基金项目： | 国家高技术研究发展计划(863计划) , 国家自然科学基金 , 国家引进国际先进农业科学技术计划(948计划) |
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| 摘 要： | 以高抗和高感黑穗病(Ustilago scitaminea Syd)的甘蔗(Saccharum complex)品种NCo376和Ya71-374为供试材料,在接种甘蔗黑穗病菌后分离叶片全蛋白,采用双向电泳技术,分析甘蔗黑穗病菌侵染后甘蔗叶片蛋白质表达谱的变化.研究结果表明,病菌侵染后,品种间、同一品种接种与对照间的叶片蛋白质表达谱均存在明显差异,部分表达上调,有些表达下调.对抗感病品种接种组中2个上调表达的蛋白质点的质谱进行进一步分析,其中一个是NBS类型的抗性蛋白,另一个是rieske Fe-S precursor protein,推测它们可能在甘蔗对黑穗病菌侵染的应答中起不同的重要作用.
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| 关 键 词： | 甘蔗 甘蔗黑穗病菌 双向电泳 串联质谱 |
Analysis of Protein Expression Patterns of Leaves of Sugarcane Infected by Ustilago scitaminea |
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| Que Youxiong,Lin Jianwei,Xu Jingsheng,Ruan Miaohong,Liao Sufeng,Xu Liping,Zhang Muqing and Chen Rukai. Analysis of Protein Expression Patterns of Leaves of Sugarcane Infected by Ustilago scitaminea[J]. Chinese Journal of Tropical Crops, 2008, 29(2): 136-140 |
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| Authors: | Que Youxiong Lin Jianwei Xu Jingsheng Ruan Miaohong Liao Sufeng Xu Liping Zhang Muqing Chen Rukai |
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| Affiliation: | Ministry of Agriculture Key Lab.of Eco-physiology & Genetic Improvement for Sugarcane;Ministry of Agriculture Key Lab.of Eco-physiology & Genetic Improvement for Sugarcane;Ministry of Agriculture Key Lab.of Eco-physiology & Genetic Improvement for Sugarcane;Ministry of Agriculture Key Lab.of Eco-physiology & Genetic Improvement for Sugarcane;Ministry of Agriculture Key Lab.of Eco-physiology & Genetic Improvement for Sugarcane;Ministry of Agriculture Key Lab.of Eco-physiology & Genetic Improvement for Sugarcane;Ministry of Agriculture Key Lab.of Eco-physiology & Genetic Improvement for Sugarcane;Ministry of Agriculture Key Lab.of Eco-physiology & Genetic Improvement for Sugarcane |
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| Abstract: | Analysis of Protein Expression Patterns of Leaves of Sugarcane Infected by Ustilago scitaminea |
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| Keywords: | sugarcane Ustilago scitaminea two dimensional electrophoresis MALDI-TOF-MS |
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