| 不同温度对马鹿精子活力的影响 |
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| 引用本文： | 骆新荣,费坤学,林敏,周伟平,高庆华.不同温度对马鹿精子活力的影响[J].经济动物学报,2012(4):192-194. |
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| 作者姓名： | 骆新荣 费坤学 林敏 周伟平 高庆华 |
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| 作者单位： | 新疆塔里木大学动物科学学院;新疆生产建设兵团塔里木畜牧科技重点实验室;新疆生产建设兵团农二师25团;新疆生产建设兵团农二师33团;新疆生产建设兵团农二师31团 |
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| 基金项目： | 兵团科技计划项目(2011BD050);塔里木大学校长基金创新群体项目(TDZKCX201201) |
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| 摘 要： | 为优选马鹿精子在流式分选前保存的方法,对6头种用马鹿麻醉电刺激采精,用改良的TRIS液稀释至精子含量为109个/mL,均分成两份,一份降温到19℃并保存18 h,另一份降温至0℃,在该温度下保存16 h,每隔2 h检测精子活率。结果表明:4 h内19℃保存条件下精子活率显著高于在0℃的,而4 h以后,在0℃保存条件下精子活率显著高于19℃的。
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| 关 键 词： | 马鹿 精子 保存方法 |
Effects of Different Temperature on Sperm Motility of Wapiti |
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| LUO Xin-rong,FEI Kun-xue,LIN Min,ZHOU Wei-ping,GAO Qing-hua.Effects of Different Temperature on Sperm Motility of Wapiti[J].Journal of Economic Animal,2012(4):192-194. |
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| Authors: | LUO Xin-rong FEI Kun-xue LIN Min ZHOU Wei-ping GAO Qing-hua |
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| Institution: | 1,2(1.College of Animal Science,Tarim University,Alaer 843300,China;2.Key Laboratory of Tarim Animal Husbandry Science and Technology,Xinjiang Production and Construction Groups,Alaer 843300,China;3.Veterinarian Station of 25st Regiment Xinjiang Production and Construction CORPS,Korla 841403,China;4.Veterinarian Station of 33st Regiment Xinjiang Production and Construction CORPS,Korla 841403,China;5.Veterinarian Station of 31st Regiment Xinjiang Production and Construction CORPS,Korla 841403,China) |
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| Abstract: | To select the best way for storage of wapiti sperm before flow cytomeric sorting,the semen was collected by electro-ejaculation from six wapiti of proven fertility.Ejaculates were diluted to the concentration of 1×109 sperm/mL with one modified TRIS extender and divided into two groups.One group was cooled to 19 ℃ and maintained at that temperature,and another was cooled to 0 ℃ and maintained at that temperature,too.The sperm motility of two groups was immediately assessed and recorded every 2 h.The sperm motility of the 19 ℃ group was significantly higher than that of the 0 ℃ group in 4 h.Four hours later,the result was contradictory(opposite). |
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| Keywords: | Cerrus elaphus sperm storage |
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